Browsing by Author "Sawanpracharak Hospital"

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    ARID1A knockdown triggers epithelial-mesenchymal transition and carcinogenesis features of renal cells: role in renal cell carcinoma
    (2019-11-01) Keerakarn Somsuan; Paleerath Peerapen; Wanida Boonmark; Sirikanya Plumworasawat; Ratirath Samol; Natthiya Sakulsak; Visith Thongboonkerd; Naresuan University; Faculty of Medicine, Siriraj Hospital, Mahidol University; Sawanpracharak Hospital
    Down-regulation/mutation of AT-rich interactive domain 1A (ARID1A), a novel tumor suppressor gene, has been reported in various cancers. Nevertheless, its role in renal cell carcinoma (RCC) remained unclear and underinvestigated. We thus evaluated carcinogenesis effects of ARID1A knockdown in nonmalignant Madin-Darby canine kidney (MDCK) renal cells using small interfering RNA (siRNA) against ARID1A (siARID1A). The siARID1A-transfected cells had decreased cell death, increased cell proliferation, and cell cycle shift (from G0/G1 to G2/M) compared with those transfected with controlled siRNA (siControl). Additionally, the siARID1A-transfected cells exhibited epithelial-mesenchymal transition (EMT) shown by greater spindle index, increased mesenchymal markers (fibronectin/vimentin), and decreased epithelial markers (E-cadherin/zonula occludens-1). Moreover, the siARID1A-transfected cells had increases in migratory activity, nuclear size, self-aggregated multicellular spheroid size, invasion capability, chemoresistance (to docetaxel), Snail family transcriptional repressor 1 expression, and TGF-β1 secretion. All of these siARID1A-knockdown effects on the carcinogenic features were reproducible in malignant RCC (786-O) cells, which exhibited a higher degree of carcinogenic phenotypes compared with the nonmalignant MDCK cells. Finally, immunohistochemistry showed obvious decrease in ARID1A protein expression in human RCC tissues (n = 23) compared with adjacent normal renal tissues (n = 23). These data indicate that ARID1A down-regulation triggers EMT and carcinogenesis features of renal cells in vitro, and its role in RCC could be proven in human tissues.-Somsuan, K., Peerapen, P., Boonmark, W., Plumworasawat, S., Samol, R., Sakulsak, N., Thongboonkerd, V. ARID1A knockdown triggers epithelial-mesenchymal transition and carcinogenesis features of renal cells: role in renal cell carcinoma.
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    Association between adenovirus infection and mortality outcome among pediatric patients after hematopoietic stem cell transplant
    (2021-12-01) Thakoon Wiriyachai; Weerapong Chaya; Usanarat Anurathapan; Sasivimol Rattanasiri; Sophida Boonsathorn; Sujittra Chaisavaneeyakorn; Chonnamet Techasaensiri; Nopporn Apiwattanakul; Faculty of Medicine Ramathibodi Hospital, Mahidol University; Sawanpracharak Hospital
    Background: Adenovirus can cause severe diseases in post-hematopoietic stem cell transplant (HSCT) patients. Because these patients also have many other factors contributing to mortality, it remains controversial whether adenovirus infection itself contributes to increased mortality in these patients. Objective: To determine if adenovirus infection contributes to mortality in pediatric post-HSCT patients. Methods: This retrospective cohort study was performed in post HSCT patients, aged 0–18 years old, admitted at Ramathibodi Hospital from 2016 to 2020. Adenovirus infection was defined as the detection of adenovirus in blood or urine by polymerase chain reaction. Multivariate cox regression was used to identify factors associated with death. Results: The incidence of overall adenovirus infection (viremia or viruria) in this cohort was 20.8% (26 out of 125 enrolled patients). From the multivariate cox regression analysis, overall adenovirus infection was not significantly associated with death (hazard ratio [HR]: 2.41; 95% confidence interval [CI]: 0.96–6.06; p =.060). However, presence of viremia (HR: 3.90; 95% CI: 1.40–10.86; p =.009), having maximal serum viral load > 10 000 copies/ml (HR: 3.70; 95% CI: 1.20–11.38; p =.023), presence of end-organ diseases (HR: 3.44; 95% CI: 1.18–10.01; p =.023) were associated with mortality. Underlying diseases requiring long-term immunosuppressive drugs before HSCT, invasive fungal disease, invasive bacterial infection, cytomegalovirus infection, and longer engraftment time were also associated with mortality. Conclusion: Overall adenovirus infection does not appear to play a significant role in mortality in pediatric post-HSCT patients. However, more invasive forms of adenovirus infection were associated with mortality in these patients.
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    Clotting tests associated with hypofibrinogenemia and systemic bleeding in green pit viper or Russell’s viper bite patients
    (2019-01-01) T. Saengnoi; P. Chantrathammachart; T. Puavilai; U. Sangchaisirisak; W. Wananukul; S. Srisuma; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Mahidol University; Sawanpracharak Hospital
    © 2019 Medical Association of Thailand. All rights reserved. Background: Serum fibrinogen of less than 100 mg/dL is recommended for predicting systemic bleeding risk in hematotoxic snake bite patients, but it is not widely available. Data on using venous clotting time (VCT), 20-minute whole blood clotting test (20WBCT), platelets, prothrombin time (PT), international normalized ratio (INR), and partial thromboplastin time (PTT) to predict systemic bleeding in hematotoxic snake bite patients are limited. Objective: To determine association between clotting tests (VCT, 20WBCT, platelets, PT, PTT, and INR) and 1) serum fibrinogen of less than 100 mg/dL, and 2) systemic bleeding in patients bitten by green pit viper (GPV) or Russell’s viper (RV). Materials and Methods: This prospective cohort study included patients bitten by GPV or RV at Sawanpracharak Hospital, Nakhon Sawan, Thailand, between October 2016 and December 2017. Patient’s blood specimens were collected for fibrinogen, PT, INR, PTT, platelet count, VCT, and 20WBCT at initial presentation and every six hours until 24 hours of admission, or patients’ recovery. The association were determined by using Fisher’s exact test. Results: There were 30 patients, 21 were bitten by GPV, and nine were bitten by RV. One hundred sixty-one sets of blood specimens were collected. There were four cases with systemic bleeding. Factors associated with fibrinogen of less than 100 mg/dL were VCT of 20 minutes or more (p=0.01), unclotted 20WBCT (p=0.01), PT of more than 13 seconds (p=0.04), and INR of 1.2 or more (p<0.01). Factors that associated with systemic bleeding were VCT of 20 minutes or more (p<0.01), unclotted 20WBCT (p<0.01), INR of 1.2 or more (p=0.04), and fibrinogen of less than 100 mg/dL (p=0.01). Conclusion: VCT of 20 minutes or more, unclotted 20WBCT, and INR of 1.2 or more are associated with serum fibrinogen less than 100 mg/dL and systemic bleeding in GPV and RV envenomation.
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    The impact of prenatal organophosphate pesticide exposures on thai infant neurodevelopment
    (2017-06-01) Pornpimol Kongtip; Benyachalee Techasaensiri; Noppanun Nankongnab; Jane Adams; Akkarat Phamonphon; Anu Surach; Supha Sangprasert; Aree Thongsuksai; Prayoon Srikumpol; Susan Woskie; Mahidol University; University of Massachusetts Boston; Sawanpracharak Hospital; Paholpolpayuhasena Hospital; Amnatcharoen Hospital; University of Massachusetts Lowell
    © 2017 by the authors. Licensee MDPI, Basel, Switzerland. A birth cohort was begun to investigate the levels and sources of pesticide exposure in pregnant women living in Thailand, and to examine the effects of pesticide exposure on infant neurodevelopment at five months of age. Subjects were interviewed using questionnaires regarding their demographic characteristics, educational background, and work and home activities related to pesticide exposures. Spot urine samples were collected at 28 weeks gestation and analyzed by gas chromatography-mass spectrometry to determine maternal metabolite levels of organophosphate pesticides including dimethyl phosphate (DMP); total DEP (diethyl phosphate (DEP), diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP), and total DAP (the sum of all metabolite levels). At five months of age, infant development was evaluated using the Bayley Scales of Infant and Toddler Development-III (Bayley-III). Higher total DEP and total DAP metabolite levels from the mother at 28 weeks’ gestation were significantly associated with reduced motor composite scores on the Bayley-III at five months of age. The total DEP levels were also significantly associated with reduced cognitive composite scores. Prenatal concentrations of maternal urinary metabolites were associated with infant cognitive and motor development. The results of several studies now suggest the need for public health intervention to reduce prenatal pesticide exposures from both agricultural and domestic use.
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    Multicenter trial of two monophasic oral contraceptives containing 30 mcg ethinylestradiol and either desogestrel or gestodene in Thai women
    (1995-01-01) Suporn Koetsawang; Chalermporn Charoenvisal; Laddawan Banharnsupawat; Suwat Singhakovin; Ouyporn Kaewsuk; Sermsak Punnahitanont; Mahidol University; Prince of Songkla University; Rajavithi Hospital; Regional Health Promotion Center; Regional Health Promotion Center; Sawanpracharak Hospital
    The efficacy and acceptability of two third generation oral contraceptives in Thai women were evaluated in a prospective, open, group-comparative, randomized, multicenter trial of women asking for contraception. In six Family Planning Centers and Outpatient Gynaecological Clinics in urban areas in Thailand, 783 healthy women who were at risk for pregnancy and did not have contraindications to oral contraceptive use were randomly allocated to one of the two study groups. An oral contraceptive containing 30 mcg ethinylestradiol and 150 mcg desogestrel was given to 394 women and an oral contraceptive with the same amount of ethinylestradiol and 75 mcg gestodene to 389 women during 6 cycles. Criteria of cycle control, side effects and the presence and severity of acne vulgaris were assessed and blood pressure and body weight measured at pretreatment and after cycles 1, 3 and 6. Furthermore, the efficacy was evaluated after the last cycle. No pregnancies occurred with either of the contraceptives. The incidences of irregular bleeding and minor side effects in both groups were very low and decreased after an initial increase in the first cycle. Acne improved in both groups. Blood pressure and body weight remained unchanged. The two oral contraceptives were found to be effective and acceptable in Thai women. Compared to Caucasian women, the incidences of irregular bleeding and side effects were apparently lower in these Asian women. Furthermore, the effects of both oral contraceptives were comparable. © 1995.
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    MYH9 disorder: Identification and a novel mutation in patients with macrothrombocytopenia
    (2021-07-01) Rungrote Natesirinilkul; Darintr Sosothikul; Patcharee Komwilaisak; Bunchoo Pongtanakul; Nattee Narkbunnum; Najwa Yudhasompop; Pimsiri Mekjarusgool; Pimjai Niparuck; Kochawan Boonyawat; Shinji Kunishima; Nongnuch Sirachainan; Somjai Kanjanapongkul; Thirachit Chotsampancharoen; Chanchai Trivaree; Siranee Wongruangsri; Pacharapan Surapolchai; Sumonmaln Klamchuen; Saranya Busakornruangrat; Kittima Kanchanakamhaeng; Nattaporntira Phalakornkul; Siriraj Hospital; Gifu University of Medical Science; Lampang Hospital; Chulalongkorn University; Hatyai Hospital; Bhumibol Adulyadej Hospital; Khon Kaen University; Faculty of Medicine Ramathibodi Hospital, Mahidol University; Maharaj Nakhon Ratchasima Hospital; Thammasat University; Queen Sirikit National Institute of Child Health; Phramongkutklao College of Medicine; Prince of Songkla University; Sawanpracharak Hospital; Chiang Mai University; Sunpasitthiprasong Hospital; Somdej Prapinklao Hospital
    The diagnosis of MYH9 disorder is guided by recognizing granulocyte Döhle body-like inclusion bodies and large/giant platelets in the peripheral blood smear. Immunofluorescence study of nonmuscle myosin heavy chain IIA is a sensitive screening method for diagnosis of MYH9 disorder. The diagnosis can then be confirmed by genetic analysis. A total of 67 patients with macrothrombocytopenia were included, of which 11 patients (16%), aged 4 months to 22 years, were ultimately diagnosed with MYH9 disorder. One novel mutation in exon 30 at c.4338T>C (p.F1446A) was detected. This mutation was associated with nonhematologic manifestations presenting in late adolescence with cataracts, hearing loss, and hematuria.
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    Nationwide surveillance and molecular characterization of critically drug-resistant gram-negative bacteria: Results of the research university network Thailand study
    (2021-09-01) Thitiya Yungyuen; Tanittha Chatsuwan; Rongpong Plongla; Sakawrat Kanthawong; Umaporn Yordpratum; Supayang P. Voravuthikunchai; Sarunyou Chusri; Dennapa Saeloh; Worada Samosornsuk; Nuntra Suwantarat; Romanee Chaiwarith; Surat Wannalerdsakun; Porpon Rotjanapan; Prawat Chantharit; Orawan Tulyaprawat; Iyarit Thaipisuttikul; Pattarachai Kiratisin; Pakpiengpen Sornwiboonsak; Nanthawadee Thongnark; Niporn Thepchai; Pattraporn Ponglorpisit; Pimpa Rungnobhakhun; Wijittraporn Onraj; Piyanat Pitakwong; Watchanarat Nitchot; Jareerat Saensud; Jintana Phimsen; Theerasak Jaifoo; Nuttiya Srisurat; Krittika Kamlangharn; Napawan Punakabutra; Sirirat Lukin; Nalinwan Wongsawat; Supat Nantasan; Kanokwan Saengsawang; Thitirat Tangkosakul; Parichat Boonrod; Nattagarn Chuenchom; Thanee Wongchai; Phadungkiat Khamnoi; Khanuengnit Nuket; Wilawan Thipmontri; Athita Riwlord; Surachai Jirapornchai; Patcharee Runapong; Sutas Boonyong; Pongpol Rerknawarat; Uraiwan Surin; Poramed Winichakoon; Pisitphon Chaimongkhol; Sarunya Srijuntongsiri; Tanatporn Chuenkeson; Patsanporn Nontaratron; Palakorn Srinitiwat; Khoriah Chedee; Vichaya Yavichai; Nutchada Tanburawong; Jirayu Visuthranukul; Umnat Klamem; Nithiarphar Bamrungsri; Pathanith Aranya; Malee Techapornrung; Sasitorn Thaicharoen; Teerarat Shanthachol; Suwimol Chooprayoon; Sarobon Chaudakshetrin; Somjit Chinapak; Narisorn Lorchirachoonkul; Puncharat Wongut-Sa; Tidarat Suttiraksa; Pachara Kasemsiri; Waranya Srichunta; Sitthipong Dumrongpiwat; Chonteera Charoenchai; Wittaya Wangsomboonsiri; Kwanjai Ketwong; Sujinda Ruangchan; Benjamat Jareeyaphadub; Sarawut Promsen; Jiraporn Nilsakul; Sunee Chayangsu; Saowarat Deekae; Huttaya Thuncharoon; Patcharee Choochur; Narissara Mungkornkaew; Chitprasong Malaisri; Yaowares Panjakun; Adisak Sangchankoom; Sarochin Pangnakorn; Tananun Tanpaibule; Daranee Nutalai; Pannawadee Uppathamnarakorn; Warattaya Ueaphathanaphanich; Nutwariya Homsukon; Narumol Boonchaiyang; Ramathibodi Hospital; Siriraj Hospital; Sukhothai Hospital; Udon Thani Center Hospital; Nakornping Hospital; Thammasat University Hospital; Lampang Hospital; Ban Pong Hospital; Prapokklao Hospital; Chulalongkorn University; Hatyai Hospital; Songkhla Hospital; Faculty of Medicine, Khon Kaen University; Faculty of Medicine, Prince of Songkia University; Trang Hospital; Police General Hospital; Bhumibol Adulyadej Hospital; Lerdsin Hospital; Surin Hospital; Naresuan University; King Chulalongkorn Memorial Hospital; Kamphaeng Phet Provincial Hospital; Vajira Hospital; Taksin Hospital; Maharaj Nakorn Chiang Mai Hospital; Thammasat University; Vichaiyut Hospital; Prince of Songkla University; Sawanpracharak Hospital; Chiang Mai University; Ratchaburi Regional Hospital; Saraburi Hospital; Wichianburi Hospital; Khon Kean Hospital; Mongkutwattana Hospital; Maharat Nakhon Ratchasima Hospital; Nakhon Pathom Hospital; Bueng Kan Hospital; Sakonnakhon Hospital; Queen Savang Vadhana Memorial Hospital; Maesot Hospital; King Narai Hospital; Angthong Hospital; Sunpasitthiprasong Hospital; Nakhon Phanom Provincial Hospital; Chum Phae Hospital; Phayao Hospital; Panyananthaphikkhu Chonprathan Medical Center; Pranangklao Hospital; Roi Et Hospital; Sawang Dandin Crown Prince Hospital; Ramkhamhaeng Hospital
    A large-scale surveillance is an important measure to monitor the regional spread of antimicrobial resistance. We prospectively studied the prevalence and molecular characteristics of clinically important Gram-negative bacilli, including Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii complex (ABC), and Pseudomonas aeruginosa, from blood, respiratory tract, urine, and sterile sites at 47 hospitals across Thailand. Among 187, 619 isolates, 93, 810 isolates (50.0%) were critically drug resistant, of which 12, 915 isolates (13.8%) were randomly selected for molecular characterization. E. coli was most commonly isolated from all specimens, except the respiratory tract, in which ABC was predominant. Prevalence of extendedspectrum cephalosporin resistance (ESCR) was higher in E. coli (42.5%) than K. pneumoniae (32.0%), but carbapenem-resistant (CR)-K. pneumoniae (17.2%) was 4.5-fold higher than CR-E. coli (3.8%). The majority of ESCR/CR-E. coli and K. pneumoniae isolates carried blaCTX-M (64.6% to 82.1%). BlaNDM and blaOXA-48-like were the most prevalent carbapenemase genes in CR-E. coli/CR-K. pneumoniae (74.9%/52.9% and 22.4%/ 54.1%, respectively). In addition, 12.9%/23.0% of CR-E. coli/CR-K. pneumoniae cocarried blaNDM and blaOXA-48-like. Among ABC isolates, 41.9% were extensively drug resistant (XDR) and 35.7% were multidrug resistant (MDR), while P. aeruginosa showed XDR/MDR at 6.3%/16.5%. A. baumannii was the most common species among ABC isolates. The major carbapenemase gene in MDR-A. baumannii/XDR-A. baumannii was blaOXA-23-like (85.8%/93.0%), which had much higher rates than other ABC species. BlaIMP, blaVIM, blaOXA-40-like, and blaOXA-58-like were also detected in ABC at lower rates. The most common carbapenemase gene in MDR/XDR-P. aeruginosa was blaIMP (29.0%/30.6%), followed by blaVIM (9.5%/25.3%). The findings reiterate an alarming situation of drug resistance that requires serious control measures.
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    Ocular injury due to potassium permanganate granules
    (2018-01-23) Chareenun Chirapapaisan; Pinnita Prabhasawat; Sabong Srivannaboon; Vatookarn Roongpoovapatr; Piyada Chitsuthipakorn; Mahidol University; Sawanpracharak Hospital; Mettapracharak (Wat Rai Khing) Hospital
    © 2018 The Author(s) Published by S. Karger AG, Basel. Purpose: We report a rare case of ocular injury due to potassium permanganate (KMnO4) granules in a child. Methods: This is a retrospective case report. Results: A 2-year-old boy was transferred to our emergency room with severe pain in his right eye, inflamed eyelids, and brownish stains on his fingers. Chemical injury was suspected. Copious eye irrigation was immediately performed. Diffuse brownish splotches were then observed at the inferior bulbar conjunctiva. Otherwise, systemic organs were intact. Complete eye exam under general anesthesia revealed a 5-mm epithelial defect at the central cornea, along with generalized conjunctival injection and limbal ischemia, inferiorly. Multiple semi-dissolved granules of KMnO4 trapped in the inferior fornix were identified. The chemical particles were gradually washed out and removed; however, the brownish stains remained. The patient received preservative-free steroid, antibiotic eye drops, and lubricants as regular management for mild to moderate degree of ocular burn. Pseudomembrane developed early and transformed into symblepharon within a few days after the injury. Membrane adhesion was lysed, and more aggressive medications were then substituted. Commercial amniotic membrane (PROKERA®) was also applied to promote wound healing and to prevent recurrence of symblepharon. The ocular surface was eventually restored, and corneal transparency was preserved. Conclusion: Ocular injury with the granular form of KMnO4 is rare. Its toxicity is comparable to concentrated KMnO4 solution. However, the dissolved particles that had been absorbed in the stained conjunctiva were continuously released and damaged the ocular surface more than we primarily anticipated. Awareness of this condition and prompt management yield a good treatment outcome.
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    Vitamin D insufficiency and deficiency among HIV-1-infected patients in a tropical setting
    (2012-09-01) Surasak Wiboonchutikul; Somnuek Sungkanuparph; Sasisopin Kiertiburanakul; La Or Chailurkit; Angkana Charoenyingwattana; Wittaya Wangsomboonsiri; Wasun Chantratita; Boonsong Ongphiphadhanakul; Mahidol University; Sawanpracharak Hospital
    Vitamin D plays role in bone health and the regulation of the immune system. A cross-sectional study of serum 25-hydroxyvitamin D (25[OH]D) levels was conducted among HIV-1-infected Thai patients to determine the prevalence and associated factors of low vitamin D levels (25[OH] D < 30 ng/mL) in tropical setting. 25-Hydroxyvitamin D was measured by liquid chromatography/tandem mass spectrometry. Of 178 patients, 58% received antiretroviral therapy at median (interquartile range [IQR]) duration of 7.4 (5.9-8.5) years. The prevalence of 25(OH)D deficiency ( < 20 ng/mL) and insufficiency (20-29.9 ng/mL) was 26.8% and 44.9%, respectively. Multivariate analysis showed that receiving efavirenz (EFV) was significantly associated with low vitamin D status (odds ratio = 3.60; 95% confidence interval, 1.06-12.15, P < .05). The mean (±standard deviation) level of 25(OH)D in patients receiving and not receiving EFV was 22.9 (6.6) and 28.6 (10.7) ng/mL, respectively, (P < .05). Low vitamin D status is common and needs to be assessed among HIV-infected patients including tropical residents especially when EFV is used. © SAGE Publications 2011.