Browsing by Author "Sercia A."

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    3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping
    (2023-03-01) Cunha D.; Souza N.; Moreira M.; Rodrigues N.; Silva P.; Franca C.; Horsophonphong S.; Sercia A.; Subbiah R.; Tahayeri A.; Ferracane J.; Yelick P.; Saboia V.; Bertassoni L.; Mahidol University
    Objectives: To develop a 3D-printed, microparticulate hydrogel supplemented with dentin matrix molecules (DMM) as a novel regenerative strategy for dental pulp capping. Materials and methods: Gelatin methacryloyl microgels (7% w/v) mixed with varying concentrations of DMM were printed using a digital light projection 3D printer and lyophilized for 2 days. The release profile of the DMM-loaded microgels was measured using a bicinchoninic acid assay. Next, dental pulp exposure defects were created in maxillary first molars of Wistar rats. The exposures were randomly capped with (1) inert material − negative control, (2) microgels, (3) microgels + DMM 500 µg/ml, (4) microgels + DMM 1000 µg/ml, (5) microgels + platelet-derived growth factor (PDGF 10 ng/ml), or (6) MTA (n = 15/group). After 4 weeks, animals were euthanized, and treated molars were harvested and then processed to evaluate hard tissue deposition, pulp tissue organization, and blood vessel density. Results: All the specimens from groups treated with microgel + 500 µg/ml, microgel + 1000 µg/ml, microgel + PDGF, and MTA showed the formation of organized pulp tissue, tertiary dentin, newly formed tubular and atubular dentin, and new blood vessel formation. Dentin bridge formation was greater and pulp necrosis was less in the microgel + DMM groups compared to MTA. Conclusions: The 3D-printed photocurable microgels doped with DMM exhibited favorable cellular and inflammatory pulp responses, and significantly more tertiary dentin deposition. Clinical relevance: 3D-printed microgel with DMM is a promising biomaterial for dentin and dental pulp regeneration in pulp capping procedures.
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    Correction to: 3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping (Clinical Oral Investigations, (2022), 27, 3, (1215-1225), 10.1007/s00784-022-04735-z)
    (2023-04-01) Cunha D.; Souza N.; Moreira M.; Rodrigues N.; Silva P.; Franca C.; Horsophonphong S.; Sercia A.; Subbiah R.; Tahayeri A.; Ferracane J.; Yelick P.; Saboia V.; Bertassoni L.; Mahidol University
    The affiliations of Dr. Horsophonphong and Dr. Bertassoni were published incorrectly: Sivaporn Horsophonphong5,6 Luiz Bertassoni8,9,10,11,12 Affiliations: 5 Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Salaya, Thailand 6 Department of Orthodontics, School of Medicine, School of Engineering, Tufts University, Boston, MA, 02,111, USA 8 Department of Orthodontics, School of Medicine, School of Engineering, Tufts University, Boston, MA, 02,111, USA 9 Center for Regenerative Medicine, School of Medicine, Oregon Health & Science University, Portland, OR, USA 10 Department of Biomedical Engineering, School of Medicine, Oregon Health & Science University, Portland, OR, USA 11 Cancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Portland, OR, USA 12 Biomaterials and Biomechanics, School of Dentistry, OR Health & Science University- OHSU, 2730 S.W. Moody Ave, Portland, OR, 97,201, USA Sivaporn Horsophonphong5 Luiz Bertassoni4,8,9,10 Affiliations 4 Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health & Science University, Portland, OR, USA 5 Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Salaya, Thailand 8 Center for Regenerative Medicine, School of Medicine, Oregon Health & Science University, Portland, OR, USA 9 Department of Biomedical Engineering, School of Medicine, Oregon Health & Science University, Portland, OR, USA 10 Cancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Portland, OR, USA The authors apologize for any inconvenience that this mistake may have caused. The original article has been corrected.
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    High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration
    (2023-01-01) Franca C.M.; Athirasala A.; Subbiah R.; Tahayeri A.; Selvakumar P.; Mansoorifar A.; Horsophonphong S.; Sercia A.; Nih L.; Bertassoni L.E.; Mahidol University
    Successful integration of cell-laden tissue constructs with host vasculature depends on the presence of functional capillaries to provide oxygen and nutrients to the embedded cells. However, diffusion limitations of cell-laden biomaterials challenge regeneration of large tissue defects that require bulk-delivery of hydrogels and cells. Here, a strategy to bioprint geometrically controlled, endothelial and stem-cell laden microgels in high-throughput is introduced, allowing these cells to form mature and functional pericyte-supported vascular capillaries in vitro, and then injecting these pre-vascularized constructs minimally invasively in-vivo. It is demonstrated that this approach offers both desired scalability for translational applications as well as unprecedented levels of control over multiple microgel parameters to design spatially-tailored microenvironments for better scaffold functionality and vasculature formation. As a proof-of-concept, the regenerative capacity of the bioprinted pre-vascularized microgels is compared with that of cell-laden monolithic hydrogels of the same cellular and matrix composition in hard-to-heal defects in vivo. The results demonstrate that the bioprinted microgels have faster and higher connective tissue formation, more vessels per area, and widespread presence of functional chimeric (human and murine) vascular capillaries across regenerated sites. The proposed strategy, therefore, addresses a significant issue in regenerative medicine, demonstrating a superior potential to facilitate translational regenerative efforts.