Journal Issue: JAAS Vol. 10 No. 3
4
Issued Date
2560
Resource Type
Language
tha
eng
eng
File Type
application/pdf
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหิดล
Journal Volume
JAAS Volume 10
(2560)
JAAS Volume 11
(2561)
Articles
Isolation and biological characterization of bacteriophages which infect Aeromonas hydrophila
(2017) Jiraporn Gatedee; Sunsiree Muangman; Pornpan Pumirat; Yuvadee Mahakunkijcharoen; Noppadol Prasertsincharoen; Kanyanan Kritsiriwuthinan; Mahidol University. Faculty of Medical Technology. Department of Microbiology and Immunology
This study aims to isolate and biological characterize bacteriophages which infect Aeromonas
hydrophila, a causative of aquatic animal diseases. Two A. hydrophila bacteriophages, øAH-S1 and øAH-T5
were isolated from a total 100 water samples collected from ponds in Bangkok, Thailand. Electron micrograph
indicated that these bacteriophages belonged to the Myoviridae family. Host-range determination revealed that
øAH-S1 and øAH-T5 have different host range patterns and capable to lyse 62.0% (18/29) and 55.1 % (16/29)
of tested A. hydrophila, respectively. The study on host-range among different species also showed different
host range patterns and unable to lyse other tested bacteria including Escherichia coli, Pseudomonas aeruginosa,
Proteus mirabilis, Vibrio cholerae and Plesiomonas spp. The efficiency to eradicate A. hydrophila ATCC 35654
in vitro showed that after 3 h of infection at multiplicity of infection 10, the reduction of average OD600 nm from
both øAH-S1and øAH-T5 infected culture was decreased to 0.095 and 0.08 respectively compared with 1.679
of non-infected control. Therefore, this study demonstrated bacteriophages which infected A. hydrophila and
these bacteriophages may be useful for future development as biocontrol agents for control of A. hydrophila
infection in aqua animals.
Development of specific activity of amylolytic and proteolytic enzymes in duodenum and jejunum of the small intestinal tract of meat-type ducks during 1-42 days of age
(2017) Kriengkrai Prahkarnkaeo; Kiattawee Choowongkomon; Boonorm Chomtee; Choawit Rakangthong; Chaiyapoom Bunchasak; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Health
This experiment was conducted to elucidate the pattern of amylolytic and proteolytic digestive enzyme
activities in meat-type ducks during 1-42 days of age. Twenty-eight male meat type ducks (Cherry Valley strain)
were used, and diets based on corn-soybean meal were offered ad lib throughout the experimental period.
At duodenal and jejunal segments, the specific activity (SA) of amylolytic (amylase) and proteolytic (trypsin,
chymotrypsin and total proteases) enzymes were determined at 1, 7, 14, 21, 28, 35 and 42 days of age. Maximal
or nearly maximal SA of all enzymes at first day after hatch (1 day of age) and the activity declined on 7 day of age
was found (P<0.05). After 7 days of age, all enzymes tended to reach a nadir at day 14 or day 21, then the SA
were significantly increased from 21 to 42 days of age (P<0.05). Therefore, the pattern of SA of all enzymes at
duodenal and jejunal segments were changed in a cubic trend (P< 0.01) with age. In ducks, it is indicated that
the embryonic enzyme reserve may be a reason of high SA of amylolytic and proteolytic enzymes at 1 day of age,
then the SA are activated again after 21 days of age by intestinal development processes and/or increasing
amount of feed intake.
การวิจัยในชั้นเรียนเพื่อสำรวจความคิดเห็นต่อการประเมินหลังการเรียนปฏิบัติการในวิชาโครงสร้างและการทำงานของร่างกายของนักศึกษาสัตวแพทย์
(2560) อาทร นกแก้ว; เติมพงศ์ วงศ์ตะวัน; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาปรีคลินิกและสัตวศาสตร์ประยุกต์; มหาวิทยาลัยมหิดล. สถาบันนวัตกรรมการเรียนรู้
This classroom action research aimed to survey the opinions of veterinary students toward a test after
finishing each laboratory class (post-test) of body structure and function courses. Second year veterinary
students of Faculty of Veterinary Science, Mahidol University from year 2014 and 2015 participated in this
survey (80 students). The tool for collecting data was online survey from, frequency and percentage of data
were analysed for rating scale questions. Responses of an open-ended question were analysed by content
analysis. The results showed that veterinary students had both positive and negative opinions about post-test.
For positive opinions, most students thought the post-test helped them to concentrate in the class and gave
them better overall score. For negative opinions, post-test caused students increased anxiety and stress due to
the format of test and unreadiness of students.
Intervention models to solve the declining problem of swamp buffalo (Bubalus bubalis) population in Ubon Ratchathani province of Thailand
(2017) Anuwat Wiratsudakul; Poonyapat Sedwisai; Teeraporn Makaroon; Sunisa Kinawong; Kittipat Angchokchatchawal; Bopit Puyati; Surapong Senayai; Mahidol University. Faculty of Veterinary Science
Swamp buffalo population in Thailand has dramatically decreased in the last three decades. The present
study was conducted to predict trends and future population size of swamp buffaloes and to formulate
different interventions to prevent further decline and rejuvenate the existing population. The study is based on a
questionnaire survey to access information regarding buffalo husbandry and marketing. Based on derived
data, age-structured Leslie matrix population model was constructed to predict the population of the buffalo
cows in the coming decades. Three different intervention models were subsequently proposed including
Buffalo bank campaign, prohibition of slaughtering buffalo cows and the combination of these two interventions.
A total of 398 farmers rearing buffaloes were interviewed and data encompassed information on 1,366 buffaloes
(418 buffalo bulls and 948 cows) were retrieved. Initial buffalo cow population in Ubon Ratchathani province
was estimated to be 42,766 heads. The number was projected to decline by 36% in the next ten years, with
population growth rate estimated to be 0.93. The most feasible and sustainable intervention was to carry out
both campaigns simultaneously. This strategy allows the slaughtering prohibition to be successful with the
support from the Buffalo bank. These campaigns should be rigorously implemented to maintain this valuable
livestock resource. The present model might be implemented for similar studies in different parts of the
developing world.
Title
JAAS Vol. 10 No. 3
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์