Publication: Isolation and biological characterization of bacteriophages which infect Aeromonas hydrophila
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2017
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eng
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1906-2257
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Mahidol University
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Faculty of Veterinary Science Mahidol University
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Journal of Applied Animal Science. Vol.10, No.3 (2017), 9-18
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Jiraporn Gatedee, Sunsiree Muangman, Pornpan Pumirat, Yuvadee Mahakunkijcharoen, Noppadol Prasertsincharoen, Kanyanan Kritsiriwuthinan Isolation and biological characterization of bacteriophages which infect Aeromonas hydrophila. Journal of Applied Animal Science. Vol.10, No.3 (2017), 9-18. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15262
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JAAS Vol. 10 No. 3
(2560)
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Isolation and biological characterization of bacteriophages which infect Aeromonas hydrophila
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การแยกและศึกษาคุณลักษณะทางชีวภาพของแบคเทอริโอฟาจของเชื้อ Aeromonas hydrophila
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This study aims to isolate and biological characterize bacteriophages which infect Aeromonas
hydrophila, a causative of aquatic animal diseases. Two A. hydrophila bacteriophages, øAH-S1 and øAH-T5
were isolated from a total 100 water samples collected from ponds in Bangkok, Thailand. Electron micrograph
indicated that these bacteriophages belonged to the Myoviridae family. Host-range determination revealed that
øAH-S1 and øAH-T5 have different host range patterns and capable to lyse 62.0% (18/29) and 55.1 % (16/29)
of tested A. hydrophila, respectively. The study on host-range among different species also showed different
host range patterns and unable to lyse other tested bacteria including Escherichia coli, Pseudomonas aeruginosa,
Proteus mirabilis, Vibrio cholerae and Plesiomonas spp. The efficiency to eradicate A. hydrophila ATCC 35654
in vitro showed that after 3 h of infection at multiplicity of infection 10, the reduction of average OD600 nm from
both øAH-S1and øAH-T5 infected culture was decreased to 0.095 and 0.08 respectively compared with 1.679
of non-infected control. Therefore, this study demonstrated bacteriophages which infected A. hydrophila and
these bacteriophages may be useful for future development as biocontrol agents for control of A. hydrophila
infection in aqua animals.