Publication:
PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction

Issued Date

2010-03-01

Resource Type

Article

ISSN

10902449
00144894

DOI

10.1016/j.exppara.2009.11.011

Other identifier(s)

2-s2.0-76049100690

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Experimental Parasitology. Vol.124, No.3 (2010), 357-359

Suggested Citation

Saiwasan Buathong, Mathirut Mungthin, Peerapan Tan-ariya, Tawee Naaglor, Paanjit Taamasri, Picha Suwannahitatorn, Saovanee Leelayoova PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction. Experimental Parasitology. Vol.124, No.3 (2010), 357-359. doi:10.1016/j.exppara.2009.11.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29254

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Title

PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction

Author(s)

Saiwasan Buathong
 Mathirut Mungthin
 Peerapan Tan-ariya
 Tawee Naaglor
 Paanjit Taamasri
 Picha Suwannahitatorn
 Saovanee Leelayoova

Other Contributor(s)

Mahidol University
 Phramongkutklao College of Medicine

Abstract

This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4°C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious. © 2009 Elsevier Inc.

Keyword(s)

Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/29254

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Scopus 2006-2010