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PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction

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dc.contributor.authorSaiwasan Buathongen_US
dc.contributor.authorMathirut Mungthinen_US
dc.contributor.authorPeerapan Tan-ariyaen_US
dc.contributor.authorTawee Naagloren_US
dc.contributor.authorPaanjit Taamasrien_US
dc.contributor.authorPicha Suwannahitatornen_US
dc.contributor.authorSaovanee Leelayoovaen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherPhramongkutklao College of Medicineen_US
dc.date.accessioned2018-09-24T09:07:17Z
dc.date.available2018-09-24T09:07:17Z
dc.date.issued2010-03-01en_US
dc.description.abstractThis study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4°C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious. © 2009 Elsevier Inc.en_US
dc.identifier.citationExperimental Parasitology. Vol.124, No.3 (2010), 357-359en_US
dc.identifier.doi10.1016/j.exppara.2009.11.011en_US
dc.identifier.issn10902449en_US
dc.identifier.issn00144894en_US
dc.identifier.other2-s2.0-76049100690en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/29254
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=76049100690&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titlePBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extractionen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=76049100690&origin=inwarden_US

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