Publication: The effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin
Issued Date
2012-01-01
Resource Type
ISSN
15734943
15723887
15723887
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2-s2.0-84859652579
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Mahidol University
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SCOPUS
Bibliographic Citation
Protein Journal. Vol.31, No.1 (2012), 43-50
Suggested Citation
Jinda Jandaruang, Jaruwan Siritapetawee, Kanjana Thumanu, Chomphunuch Songsiriritthigul, Chartchai Krittanai, Sakda Daduang, Apisak Dhiravisit, Sompong Thammasirirak The effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin. Protein Journal. Vol.31, No.1 (2012), 43-50. doi:10.1007/s10930-011-9372-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13865
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Title
The effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin
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Abstract
Crocodylus siamensis hemoglobin (cHb) was purified by gel filtration chromatography and visualized by SDS-PAGE. Effects of temperature and pH on secondary structure and conformation changes of cHb were studied using circular dichroism spectropolarimeter and fourier transform infrared spectrophotometer. The secondary structure of intact cHb was mainly α-helices. cHb was not heat stable when heated at 65 °C and cooled down to original temperature, indicating the irreversible unfolding process. The stability of cHb at different pH ranging from 2.5 to 10.5 was determined. The maximum value of the α-helix content was found at pH 3.5 and tended to decrease at strong acid and strong base. The antioxidant activities of heat treated cHb and cHb in solution with pH range 2.5 to 10.5 were tested by DPPH radical scavenging assay. cHb at pH 4.5, having highest β-turn structure, showed highest radical scavenging activity. In contrast to pH, heat had no effect on antioxidant activity of cHb. © Springer Science+Business Media, LLC 2011.