Publication:
The effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin

Simple item page
dc.contributor.authorJinda Jandaruangen_US
dc.contributor.authorJaruwan Siritapetaweeen_US
dc.contributor.authorKanjana Thumanuen_US
dc.contributor.authorChomphunuch Songsiriritthigulen_US
dc.contributor.authorChartchai Krittanaien_US
dc.contributor.authorSakda Daduangen_US
dc.contributor.authorApisak Dhiravisiten_US
dc.contributor.authorSompong Thammasiriraken_US
dc.contributor.otherKhon Kaen Universityen_US
dc.contributor.otherSuranaree University of Technologyen_US
dc.contributor.otherPublic Organizationen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-06-11T04:40:38Z
dc.date.available2018-06-11T04:40:38Z
dc.date.issued2012-01-01en_US
dc.description.abstractCrocodylus siamensis hemoglobin (cHb) was purified by gel filtration chromatography and visualized by SDS-PAGE. Effects of temperature and pH on secondary structure and conformation changes of cHb were studied using circular dichroism spectropolarimeter and fourier transform infrared spectrophotometer. The secondary structure of intact cHb was mainly α-helices. cHb was not heat stable when heated at 65 °C and cooled down to original temperature, indicating the irreversible unfolding process. The stability of cHb at different pH ranging from 2.5 to 10.5 was determined. The maximum value of the α-helix content was found at pH 3.5 and tended to decrease at strong acid and strong base. The antioxidant activities of heat treated cHb and cHb in solution with pH range 2.5 to 10.5 were tested by DPPH radical scavenging assay. cHb at pH 4.5, having highest β-turn structure, showed highest radical scavenging activity. In contrast to pH, heat had no effect on antioxidant activity of cHb. © Springer Science+Business Media, LLC 2011.en_US
dc.identifier.citationProtein Journal. Vol.31, No.1 (2012), 43-50en_US
dc.identifier.doi10.1007/s10930-011-9372-7en_US
dc.identifier.issn15734943en_US
dc.identifier.issn15723887en_US
dc.identifier.other2-s2.0-84859652579en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/13865
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859652579&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemical Engineeringen_US
dc.subjectChemistryen_US
dc.titleThe effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobinen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859652579&origin=inwarden_US

Files

Collections

Scopus 2011-2015