Publication:
Multiple populations of artemisinin-resistant Plasmodium falciparum in Cambodia

Issued Date

2013-06-01

Resource Type

Article

ISSN

15461718
10614036

DOI

10.1038/ng.2624

Other identifier(s)

2-s2.0-84878715473

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Nature Genetics. Vol.45, No.6 (2013), 648-655

Suggested Citation

Olivo Miotto, Jacob Almagro-Garcia, Magnus Manske, Bronwyn MacInnis, Susana Campino, Kirk A. Rockett, Chanaki Amaratunga, Pharath Lim, Seila Suon, Sokunthea Sreng, Jennifer M. Anderson, Socheat Duong, Chea Nguon, Char Meng Chuor, David Saunders, Youry Se, Chantap Lon, Mark M. Fukuda, Lucas Amenga-Etego, Abraham V.O. Hodgson, Victor Asoala, Mallika Imwong, Shannon Takala-Harrison, François Nosten, Xin Zhuan Su, Pascal Ringwald, Frédéric Ariey, Christiane Dolecek, Tran Tinh Hien, Maciej F. Boni, Cao Quang Thai, Alfred Amambua-Ngwa, David J. Conway, Abdoulaye A. Djimdé, Ogobara K. Doumbo, Issaka Zongo, Jean Bosco Ouedraogo, Daniel Alcock, Eleanor Drury, Sarah Auburn, Oliver Koch, Mandy Sanders, Christina Hubbart, Gareth Maslen, Valentin Ruano-Rubio, Dushyanth Jyothi, Alistair Miles, John O'Brien, Chris Gamble, Samuel O. Oyola, Julian C. Rayner, Chris I. Newbold, Matthew Berriman, Chris C.A. Spencer, Gilean McVean, Nicholas P. Day, Nicholas J. White, Delia Bethell, Arjen M. Dondorp, Christopher V. Plowe, Rick M. Fairhurst, Dominic P. Kwiatkowski Multiple populations of artemisinin-resistant Plasmodium falciparum in Cambodia. Nature Genetics. Vol.45, No.6 (2013), 648-655. doi:10.1038/ng.2624 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31306

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Title

Multiple populations of artemisinin-resistant Plasmodium falciparum in Cambodia

Author(s)

Olivo Miotto
 Jacob Almagro-Garcia
 Magnus Manske
 Bronwyn MacInnis
 Susana Campino
 Kirk A. Rockett
 Chanaki Amaratunga
 Pharath Lim
 Seila Suon
 Sokunthea Sreng
 Jennifer M. Anderson
 Socheat Duong
 Chea Nguon
 Char Meng Chuor
 David Saunders
 Youry Se
 Chantap Lon
 Mark M. Fukuda
 Lucas Amenga-Etego
 Abraham V.O. Hodgson
 Victor Asoala
 Mallika Imwong
 Shannon Takala-Harrison
 François Nosten
 Xin Zhuan Su
 Pascal Ringwald
 Frédéric Ariey
 Christiane Dolecek
 Tran Tinh Hien
 Maciej F. Boni
 Cao Quang Thai
 Alfred Amambua-Ngwa
 David J. Conway
 Abdoulaye A. Djimdé
 Ogobara K. Doumbo
 Issaka Zongo
 Jean Bosco Ouedraogo
 Daniel Alcock
 Eleanor Drury
 Sarah Auburn
 Oliver Koch
 Mandy Sanders
 Christina Hubbart
 Gareth Maslen
 Valentin Ruano-Rubio
 Dushyanth Jyothi
 Alistair Miles
 John O'Brien
 Chris Gamble
 Samuel O. Oyola
 Julian C. Rayner
 Chris I. Newbold
 Matthew Berriman
 Chris C.A. Spencer
 Gilean McVean
 Nicholas P. Day
 Nicholas J. White
 Delia Bethell
 Arjen M. Dondorp
 Christopher V. Plowe
 Rick M. Fairhurst
 Dominic P. Kwiatkowski

Other Contributor(s)

University of Oxford
 Mahidol University
 Wellcome Trust Sanger Institute
 Wellcome Trust Centre for Human Genetics
 National Institute of Allergy and Infectious Diseases
 National Center for Parasitology, Entomology and Malaria Control
 Armed Forces Research Institute of Medical Sciences, Thailand
 USAMC-AFRIMS
 Armed Forces Health Surveillance Center
 Navrongo Health Research Center
 University of Maryland School of Medicine
 Shoklo Malaria Research Unit
 Organisation Mondiale de la Sante
 Institut Pasteur, Paris
 Oxford University Clinical Research Unit
 Medical Research Council Laboratories Gambia
 London School of Hygiene & Tropical Medicine
 University of Sciences
 Institut de Recherche en Sciences de la Santé
 Menzies School of Health Research
 Weatherall Institute of Molecular Medicine

Abstract

We describe an analysis of genome variation in 825 P. falciparum samples from Asia and Africa that identifies an unusual pattern of parasite population structure at the epicenter of artemisinin resistance in western Cambodia. Within this relatively small geographic area, we have discovered several distinct but apparently sympatric parasite subpopulations with extremely high levels of genetic differentiation. Of particular interest are three subpopulations, all associated with clinical resistance to artemisinin, which have skewed allele frequency spectra and high levels of haplotype homozygosity, indicative of founder effects and recent population expansion. We provide a catalog of SNPs that show high levels of differentiation in the artemisinin-resistant subpopulations, including codon variants in transporter proteins and DNA mismatch repair proteins. These data provide a population-level genetic framework for investigating the biological origins of artemisinin resistance and for defining molecular markers to assist in its elimination. © 2013 Nature America, Inc. All rights reserved.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/31306

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