The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 18-20
Suggested Citation
Charoonluk Sangloung, Ruangrat Buddhirongawatr, Nitipan Tantawiwattananon, Yaowalark Sukthana In vivo culture of Cryptosporidium oocysts for laboratory use.. The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 18-20. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23421
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In vivo culture of Cryptosporidium oocysts for laboratory use.
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.
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