Publication: In vivo culture of Cryptosporidium oocysts for laboratory use.
| dc.contributor.author | Charoonluk Sangloung | en_US |
| dc.contributor.author | Ruangrat Buddhirongawatr | en_US |
| dc.contributor.author | Nitipan Tantawiwattananon | en_US |
| dc.contributor.author | Yaowalark Sukthana | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-08-20T07:05:12Z | |
| dc.date.available | 2018-08-20T07:05:12Z | |
| dc.date.issued | 2006-12-01 | en_US |
| dc.description.abstract | In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use. | en_US |
| dc.identifier.citation | The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 18-20 | en_US |
| dc.identifier.issn | 01251562 | en_US |
| dc.identifier.other | 2-s2.0-34447550597 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/23421 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34447550597&origin=inward | en_US |
| dc.subject | Medicine | en_US |
| dc.title | In vivo culture of Cryptosporidium oocysts for laboratory use. | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34447550597&origin=inward |