Publication:
Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A

Issued Date

2021-08-01

Resource Type

Article

ISSN

18767753
18735061

DOI

10.1016/j.scr.2021.102472

Other identifier(s)

2-s2.0-85111051321

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Stem Cell Research. Vol.55, (2021)

Suggested Citation

Phatchanat Klaihmon, Sudjit Luanpitpong, Chanchao Lorthongpanich, Chuti Laowtammathron, Supaporn Waeteekul, Papussorn Terbto, Surapol Issaragrisil Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A. Stem Cell Research. Vol.55, (2021). doi:10.1016/j.scr.2021.102472 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76092

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A

Author(s)

Phatchanat Klaihmon
 Sudjit Luanpitpong
 Chanchao Lorthongpanich
 Chuti Laowtammathron
 Supaporn Waeteekul
 Papussorn Terbto
 Surapol Issaragrisil

Other Contributor(s)

Siriraj Hospital

Abstract

Natural killer (NK) cells were isolated from human umbilical cord blood from a healthy newborn and reprogrammed by episomal vectors carrying reprograming factors L-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53 delivered using nucleofection. The obtained MUSIi013-A human induced pluripotent stem cell (iPSC) line highly expressed pluripotency markers, had the capacity to differentiate into derivatives of the three germ layers, while retained a normal karyotype. This cell line may be a useful tool to study epigenic memory that may predispose hiPSCs to enhanced NK differentiation.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/76092

Collections

Scopus 2021