Publication: Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells
Issued Date
2013-06-01
Resource Type
ISSN
18727492
01681702
01681702
Other identifier(s)
2-s2.0-84877624305
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Mahidol University
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SCOPUS
Bibliographic Citation
Virus Research. Vol.174, No.1-2 (2013), 37-46
Suggested Citation
Sutha Sangiambut, Amporn Suphatrakul, Rungtawan Sriburi, Poonsook Keelapang, Chunya Puttikhunt, Watchara Kasinrerk, Prida Malasit, Nopporn Sittisombut Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells. Virus Research. Vol.174, No.1-2 (2013), 37-46. doi:10.1016/j.virusres.2013.02.009 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31304
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Title
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells
Abstract
A simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells. © 2013 Elsevier B.V.