Publication:
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells

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dc.contributor.authorSutha Sangiambuten_US
dc.contributor.authorAmporn Suphatrakulen_US
dc.contributor.authorRungtawan Sriburien_US
dc.contributor.authorPoonsook Keelapangen_US
dc.contributor.authorChunya Puttikhunten_US
dc.contributor.authorWatchara Kasinrerken_US
dc.contributor.authorPrida Malasiten_US
dc.contributor.authorNopporn Sittisombuten_US
dc.contributor.otherThailand National Center for Genetic Engineering and Biotechnologyen_US
dc.contributor.otherChiang Mai Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-10-19T04:39:12Z
dc.date.available2018-10-19T04:39:12Z
dc.date.issued2013-06-01en_US
dc.description.abstractA simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells. © 2013 Elsevier B.V.en_US
dc.identifier.citationVirus Research. Vol.174, No.1-2 (2013), 37-46en_US
dc.identifier.doi10.1016/j.virusres.2013.02.009en_US
dc.identifier.issn18727492en_US
dc.identifier.issn01681702en_US
dc.identifier.other2-s2.0-84877624305en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/31304
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877624305&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleSustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cellsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877624305&origin=inwarden_US

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