Publication: Determination of suppressive effect on human T-cell activation by hispidulin, nepetin, and vanillic acid
Issued Date
2019-11-02
Resource Type
ISSN
15322513
08923973
08923973
Other identifier(s)
2-s2.0-85074481217
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Immunopharmacology and Immunotoxicology. Vol.41, No.6 (2019), 591-598
Suggested Citation
Premrutai Thitilertdecha, Varangkana Tantithavorn, Poonsin Poungpairoj, Nattawat Onlamoon Determination of suppressive effect on human T-cell activation by hispidulin, nepetin, and vanillic acid. Immunopharmacology and Immunotoxicology. Vol.41, No.6 (2019), 591-598. doi:10.1080/08923973.2019.1675165 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50995
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Determination of suppressive effect on human T-cell activation by hispidulin, nepetin, and vanillic acid
Other Contributor(s)
Abstract
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. Background: Hispidulin, nepetin, and vanillic acid are phenolic compounds potentially possessing immunosuppressive property, however, no information on their pharmacological effect and cytotoxicity has been investigated on human T lymphocytes. Materials and methods: Human peripheral blood mononuclear cells were stimulated with anti-CD3/28 coated beads and treated with individual compound at different concentrations (50–200 µM). Inhibition of early cell activation and induction of apoptosis were analyzed by flow cytometric technique. Results: At 200 µM, frequencies of CD25 and CD69 in CD4+ and CD8+ T lymphocytes were markedly decreased by hispidulin and nepetin. When lowering to 100 and 50 µM, hispidulin had no effect on the expression of CD69 in CD4+ T cells, whereas nepetin selectively suppressed CD25 and CD69 expressions in CD8+ T cells at 100 µM and only inhibited CD69 in CD8+ T cells at 50 µM. For vanillic acid, no inhibitory effect was observed while cell activation was significantly increased for all treated concentrations. None of these compounds disturbed levels of total apoptotic cells in CD4+ and CD8+ populations. Conclusions: Hispidulin and nepetin, therefore, exhibit dose-dependent inhibitory activity of early T-cell activation without inducing cell death, considering feasible immunosuppressants for inflammation-related diseases. However, vanillic acid has no effect on immunosuppression but shows more potential on immunostimulation.Highlights Immunosuppressive effects of hispidulin and nepetin on human T cells were studied. Dose-dependent activity for T-cell suppression was found in hispidulin and nepetin. Vanillic acid showed immunostimulating potential rather than immunosuppression. All compounds did not induce cell death.