Publication:
Determination of suppressive effect on human T-cell activation by hispidulin, nepetin, and vanillic acid

dc.contributor.authorPremrutai Thitilertdechaen_US
dc.contributor.authorVarangkana Tantithavornen_US
dc.contributor.authorPoonsin Poungpairojen_US
dc.contributor.authorNattawat Onlamoonen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.date.accessioned2020-01-27T08:51:49Z
dc.date.available2020-01-27T08:51:49Z
dc.date.issued2019-11-02en_US
dc.description.abstract© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. Background: Hispidulin, nepetin, and vanillic acid are phenolic compounds potentially possessing immunosuppressive property, however, no information on their pharmacological effect and cytotoxicity has been investigated on human T lymphocytes. Materials and methods: Human peripheral blood mononuclear cells were stimulated with anti-CD3/28 coated beads and treated with individual compound at different concentrations (50–200 µM). Inhibition of early cell activation and induction of apoptosis were analyzed by flow cytometric technique. Results: At 200 µM, frequencies of CD25 and CD69 in CD4+ and CD8+ T lymphocytes were markedly decreased by hispidulin and nepetin. When lowering to 100 and 50 µM, hispidulin had no effect on the expression of CD69 in CD4+ T cells, whereas nepetin selectively suppressed CD25 and CD69 expressions in CD8+ T cells at 100 µM and only inhibited CD69 in CD8+ T cells at 50 µM. For vanillic acid, no inhibitory effect was observed while cell activation was significantly increased for all treated concentrations. None of these compounds disturbed levels of total apoptotic cells in CD4+ and CD8+ populations. Conclusions: Hispidulin and nepetin, therefore, exhibit dose-dependent inhibitory activity of early T-cell activation without inducing cell death, considering feasible immunosuppressants for inflammation-related diseases. However, vanillic acid has no effect on immunosuppression but shows more potential on immunostimulation.Highlights Immunosuppressive effects of hispidulin and nepetin on human T cells were studied. Dose-dependent activity for T-cell suppression was found in hispidulin and nepetin. Vanillic acid showed immunostimulating potential rather than immunosuppression. All compounds did not induce cell death.en_US
dc.identifier.citationImmunopharmacology and Immunotoxicology. Vol.41, No.6 (2019), 591-598en_US
dc.identifier.doi10.1080/08923973.2019.1675165en_US
dc.identifier.issn15322513en_US
dc.identifier.issn08923973en_US
dc.identifier.other2-s2.0-85074481217en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/50995
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074481217&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleDetermination of suppressive effect on human T-cell activation by hispidulin, nepetin, and vanillic aciden_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074481217&origin=inwarden_US

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