Publication: Induction of human cholesterol 7α-hydroxylase in HepG2 cells by 2,4,6-trihydroxyacetophenone
Issued Date
2005-05-16
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ISSN
00142999
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2-s2.0-20344362658
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Mahidol University
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SCOPUS
Bibliographic Citation
European Journal of Pharmacology. Vol.515, No.1-3 (2005), 43-46
Suggested Citation
Juree Charoenteeraboon, Kasem Nithipatikom, William B. Campbell, Pawinee Piyachaturawat, Prapon Wilairat, Pornpimol Rongnoparut Induction of human cholesterol 7α-hydroxylase in HepG2 cells by 2,4,6-trihydroxyacetophenone. European Journal of Pharmacology. Vol.515, No.1-3 (2005), 43-46. doi:10.1016/j.ejphar.2005.03.039 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17146
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Title
Induction of human cholesterol 7α-hydroxylase in HepG2 cells by 2,4,6-trihydroxyacetophenone
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Abstract
In animal the plasma cholesterol-lowering activity of 2,4,6- trihydroxyacetophenone (THA) is due to enhanced cholesterol 7α-hydroxylase (CYP7A1) activity. We have examined the effect of THA on CYP7A1 activity and mRNA level in HepG2 cells. THA stimulated CYP7A1 activity in a concentration- and time-dependent manner. After exposure for 24 h, 1 μM THA induced CYP7A1 activity 160 ± 8% and mRNA level 166 ± 21% (mean ± S.E.M.) of control. Moreover THA antagonized the inhibitory regulation of chenodeoxycholic acid on CYP7A1 mRNA expression. These results indicated that THA increases CYP7A1 activity in human HepG2 cells by stimulating mRNA transcription. © 2005 Elsevier B.V. All rights reserved.