Publication: Epithelial-specific SHP1-P2 methylation - A novel universal tumor marker for detection of colorectal cancer lymph node metastasis
Issued Date
2016-01-01
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ISSN
2476762X
15137368
15137368
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2-s2.0-84988443671
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.17, No.8 (2016), 4119-4125
Suggested Citation
Prakasit Rattanatanyong, Somboon Keelawat, Nakarin Kitkumthorn, Apiwat Mutirangura Epithelial-specific SHP1-P2 methylation - A novel universal tumor marker for detection of colorectal cancer lymph node metastasis. Asian Pacific Journal of Cancer Prevention. Vol.17, No.8 (2016), 4119-4125. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43213
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Title
Epithelial-specific SHP1-P2 methylation - A novel universal tumor marker for detection of colorectal cancer lymph node metastasis
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Abstract
Background: Methylation of promoter 2 of the SHP1 gene is epithelial cell specific, with reported potential as a lymph node metastatic marker. Objective: To demonstrate SHP1-P2 methylation-specific quantitative PCR effectiveness in detecting colorectal cancer (CRC) DNA in lymph nodes. Materials and Methods: SHP1-P2 methylation levels were measured in lymph nodes of CRC patients and compared with pathological findings and patient prognosis. Results: Lymph nodes of CRC metastatic patients without microscopically detectable cancer cells had higher SHP1-P2 methylation levels than lymph nodes of controls (p < 0.001). In addition, a higher SHP1-P2 methylation level was associated with a shorter duration until adverse disease events, metastasis, recurrence and death (r2 = 0.236 and p value = 0.048). Studying two cohorts of 74 CRC patients without microscopic lymph node metastases showed that only the cohort containing samples with high SHP1-P2 methylation levels had a significant hazard ratio of 3.8 (95%CI = 1.02 to 14.2). Conclusions: SHP1-P2 methylation PCR can detectCRC cancer DNA in lymph nodes even if cancer cells are not visible under a microscope, confirming its' potential universal lymph node metastatic marker.