Publication: Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples
item.page.dc.date.issued
2007-09-21
item.page.dc.type
item.page.dc.identifier.issn
07317085
item.page.dc.identifier.doi
item.page.dc.identifier.other
2-s2.0-34548474579
item.page.dc.rights
Mahidol University
item.page.dc.rights.holder
SCOPUS
item.page.dc.identifier.citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.45, No.1 (2007), 149-153
punsarn.item.page.citation
N. Lindegårdh, A. M. Dondorp, P. Singhasivanon, N. J. White, N. P J Day Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Journal of Pharmaceutical and Biomedical Analysis. Vol.45, No.1 (2007), 149-153. doi:10.1016/j.jpba.2007.04.030 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24336
Research Projects
Organizational Units
Authors
Journal Issue
relationships.isThesisOf
item.page.dc.title
Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples
item.page.dc.contributor.author
item.page.dc.contributor.other
item.page.dc.description.abstract
A simple and rapid liquid chromatographic-mass spectrometric assay for the evaluation of artesunate in vials for injection has been developed and validated. The content of each vial was dissolved in 3.0 mL of methanol using a SGE analytical syringe (1.0 mL). Each sample was diluted to a theoretical concentration of 1000 ng/mL and analysed in triplicate. Three replicates of calibration standards at concentrations 500, 1000 and 1500 ng/mL were used to construct a calibration curve. Artesunate was analysed by liquid chromatography with atmospheric pressure chemical ionisation (APCI) mass spectrometric (MS) detection on a Hypersil Gold column (100 mm × 4.6 mm) using a mobile phase containing methanol-ammonium acetate 10 mM pH 5.3 (70:30, v/v) at a flow rate of 1 mL/min. The assay was implemented for the analysis of artesunate for injection purchased from Guilin Pharmaceutical Company in China. © 2007 Elsevier B.V. All rights reserved.