Publication: Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples
Issued Date
2007-09-21
Resource Type
ISSN
07317085
Other identifier(s)
2-s2.0-34548474579
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.45, No.1 (2007), 149-153
Suggested Citation
N. Lindegårdh, A. M. Dondorp, P. Singhasivanon, N. J. White, N. P J Day Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Journal of Pharmaceutical and Biomedical Analysis. Vol.45, No.1 (2007), 149-153. doi:10.1016/j.jpba.2007.04.030 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24336
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Title
Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples
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Abstract
A simple and rapid liquid chromatographic-mass spectrometric assay for the evaluation of artesunate in vials for injection has been developed and validated. The content of each vial was dissolved in 3.0 mL of methanol using a SGE analytical syringe (1.0 mL). Each sample was diluted to a theoretical concentration of 1000 ng/mL and analysed in triplicate. Three replicates of calibration standards at concentrations 500, 1000 and 1500 ng/mL were used to construct a calibration curve. Artesunate was analysed by liquid chromatography with atmospheric pressure chemical ionisation (APCI) mass spectrometric (MS) detection on a Hypersil Gold column (100 mm × 4.6 mm) using a mobile phase containing methanol-ammonium acetate 10 mM pH 5.3 (70:30, v/v) at a flow rate of 1 mL/min. The assay was implemented for the analysis of artesunate for injection purchased from Guilin Pharmaceutical Company in China. © 2007 Elsevier B.V. All rights reserved.