Publication: Mass propagation of kaempferia parviflora wall. ex baker by in vitro regeneration
Issued Date
2007-01-01
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ISSN
14620316
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2-s2.0-75849159244
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Horticultural Science and Biotechnology. Vol.82, No.2 (2007), 179-183
Suggested Citation
S. Prathanturarug, T. Apichartbutra, W. Chuakul, P. Saralamp Mass propagation of kaempferia parviflora wall. ex baker by in vitro regeneration. Journal of Horticultural Science and Biotechnology. Vol.82, No.2 (2007), 179-183. doi:10.1080/14620316.2007.11512217 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24042
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Title
Mass propagation of kaempferia parviflora wall. ex baker by in vitro regeneration
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Abstract
An efficient micropropagation protocol for a potential medicinal plant, Kaempferia parviflora Wall, ex Baker, was established. We investigated the effects of plant growth regulators (PGRs) and the duration of PGR treatment on shoot regeneration. Terminal buds were cultured on Murashige and Skoog (MS) medium supplemented with 35.52 uM 6-benzylaminopurine for 8 weeks then transferred to PGR-free MS for a further 4 weeks. After a second cycle, we obtained 47.3 ± 3.4 shoots per initial explant, after a total of 24 weeks of culture. Rooting was spontaneous. Approx. 20,000 regenerants were successfully transferred to soil and subsequently grown in the field.