Publication: A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction
Issued Date
1995-01-01
Resource Type
ISSN
08908508
Other identifier(s)
2-s2.0-0029587426
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Cellular Probes. Vol.9, No.6 (1995), 375-382
Suggested Citation
Sarawut Jitrapakdee, Anchalee Tassanakajon, Vichai Boonsaeng, Suwit Piankijagum, Sakol Panyim A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction. Molecular and Cellular Probes. Vol.9, No.6 (1995), 375-382. doi:10.1006/mcpr.1995.0059 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17253
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Title
A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction
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Abstract
A polymerase chain reaction (PCR) method was developed for detection of salmonella in food. A set of PCR primers was designed to amplify a 199 bp salmonella-specific DNA fragment derived from a repetitive DNA of Salmonella Weltevreden. The assay detected all 52 most prevalent serovars found in contaminated food in Thailand and no cross-reaction was observed with other non-salmonella organisms. The limit of detection was 1 fg of purified target DNA or five bacteria from pure culture. The detection of artificially contaminated food performed following a 6 h enrichment step was three bacteria per gram and the result was obtained within 4 h. © 1995 Academic Press Limited.