Publication: A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction
| dc.contributor.author | Sarawut Jitrapakdee | en_US |
| dc.contributor.author | Anchalee Tassanakajon | en_US |
| dc.contributor.author | Vichai Boonsaeng | en_US |
| dc.contributor.author | Suwit Piankijagum | en_US |
| dc.contributor.author | Sakol Panyim | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Chulalongkorn University | en_US |
| dc.date.accessioned | 2018-07-04T06:52:21Z | |
| dc.date.available | 2018-07-04T06:52:21Z | |
| dc.date.issued | 1995-01-01 | en_US |
| dc.description.abstract | A polymerase chain reaction (PCR) method was developed for detection of salmonella in food. A set of PCR primers was designed to amplify a 199 bp salmonella-specific DNA fragment derived from a repetitive DNA of Salmonella Weltevreden. The assay detected all 52 most prevalent serovars found in contaminated food in Thailand and no cross-reaction was observed with other non-salmonella organisms. The limit of detection was 1 fg of purified target DNA or five bacteria from pure culture. The detection of artificially contaminated food performed following a 6 h enrichment step was three bacteria per gram and the result was obtained within 4 h. © 1995 Academic Press Limited. | en_US |
| dc.identifier.citation | Molecular and Cellular Probes. Vol.9, No.6 (1995), 375-382 | en_US |
| dc.identifier.doi | 10.1006/mcpr.1995.0059 | en_US |
| dc.identifier.issn | 08908508 | en_US |
| dc.identifier.other | 2-s2.0-0029587426 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/17253 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0029587426&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.title | A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0029587426&origin=inward | en_US |