Publication: Toxoplasma gondii infection: What is the real situation?
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Issued Date
2013-12-01
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ISSN
10902449
00144894
00144894
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2-s2.0-84887524600
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.135, No.4 (2013), 685-689
Suggested Citation
Waenurama Chemoh, Nongyao Sawangjaroen, Veeranoot Nissapatorn, Chitkasaem Suwanrath, Verapol Chandeying, Thanaporn Hortiwakul, Hemah Andiappan, Natthawan Sermwittayawong, Bunsri Charoenmak, Pisut Siripaitoon, Amorn Lekkla, Yaowalark Sukthana Toxoplasma gondii infection: What is the real situation?. Experimental Parasitology. Vol.135, No.4 (2013), 685-689. doi:10.1016/j.exppara.2013.10.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31823
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Title
Toxoplasma gondii infection: What is the real situation?
Abstract
The prevalence of chronic Toxoplasma infections reported in the literature varies enormously. We hypothesize that one factor could be due to the different methods used in the evaluation of infections. Serological evidence of Toxoplasma infections in 450 pregnant women (PW) and 300 HIV-infected patients (HIV) were investigated by the Sabin-Feldman dye test and two other commercial ELISA kits (kit1 and kit2). Anti Toxoplasma IgG antibodies obtained from the Sabin-Feldman dye test, ELISA kit1 and ELISA kit2 in the PW subjects were 14.7%, 29.6% and 38.7%, and in the HIV subjects were 13%, 34.7% and 36.3%, respectively. So there were significant differences in the seroprevalences when different diagnostic tests were used (P<. 0.05). Regarding Sabin-Feldman dye test as the gold standard for anti Toxoplasma antibodies detection, we found that the sensitivity and specificity of the ELISA kit1 and kit2 was in the range of their specification. However as the two ELISA kits used in our study identified a much higher prevalence of Toxoplasma infections which indicated that false positive cases were being reported. Based on results obtained, it is therefore highly recommended that research workers should be aware that the reports of serological studies in terms of high positive results should be treated with some skepticism until additional precise diagnostic tools are developed. © 2013 Elsevier Inc.