Publication: Immobilization-free electrochemical sensor coupled with a graphene-oxide-based aptasensor for glycated albumin detection
Issued Date
2021-03-01
Resource Type
ISSN
20796374
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2-s2.0-85103919871
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Mahidol University
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SCOPUS
Bibliographic Citation
Biosensors. Vol.11, No.3 (2021)
Suggested Citation
Wassa Waiwinya, Thitirat Putnin, Dechnarong Pimalai, Wireeya Chawjiraphan, Nuankanya Sathirapongsasuti, Deanpen Japrung Immobilization-free electrochemical sensor coupled with a graphene-oxide-based aptasensor for glycated albumin detection. Biosensors. Vol.11, No.3 (2021). doi:10.3390/bios11030085 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76256
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Title
Immobilization-free electrochemical sensor coupled with a graphene-oxide-based aptasensor for glycated albumin detection
Abstract
An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.