Publication:
High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity

Issued Date

1992-11-06

Resource Type

Article

ISSN

03784347

DOI

10.1016/0378-4347(92)80302-7

Other identifier(s)

2-s2.0-0026455012

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Chromatography B: Biomedical Sciences and Applications. Vol.582, No.1-2 (1992), 57-64

Suggested Citation

Isra Ittarat, H. Kyle Webster, Yongyuth Yuthavong High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity. Journal of Chromatography B: Biomedical Sciences and Applications. Vol.582, No.1-2 (1992), 57-64. doi:10.1016/0378-4347(92)80302-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22252

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Title

High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity

Author(s)

Isra Ittarat
 H. Kyle Webster
 Yongyuth Yuthavong

Other Contributor(s)

Mahidol University
 Armed Forces Research Institute of Medical Sciences, Thailand

Abstract

A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gametocyte stages of P. falciparum in culture with menoctone, primaquine or the primaquine derivative WR 238605 led to depression of the enzyme activity, although the drugs did not appear to inhibit the enzyme directly. © 1992.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Chemistry

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/22252

Collections

Scopus 1991-2000