Publication: Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034
Issued Date
2016-03-01
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ISSN
14320614
01757598
01757598
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2-s2.0-84959162007
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Mahidol University
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SCOPUS
Bibliographic Citation
Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-2760
Suggested Citation
Bungonsiri Intra, Jirayut Euanorasetr, Takuya Nihira, Watanalai Panbangred Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034. Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-2760. doi:10.1007/s00253-015-7142-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43090
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Title
Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034
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Abstract
© 2015, Springer-Verlag Berlin Heidelberg. Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034.