Publication:
Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034

Issued Date

2016-03-01

Resource Type

Article

ISSN

14320614
01757598

DOI

10.1007/s00253-015-7142-8

Other identifier(s)

2-s2.0-84959162007

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-2760

Suggested Citation

Bungonsiri Intra, Jirayut Euanorasetr, Takuya Nihira, Watanalai Panbangred Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034. Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-2760. doi:10.1007/s00253-015-7142-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43090

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Title

Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034

Author(s)

Bungonsiri Intra
 Jirayut Euanorasetr
 Takuya Nihira
 Watanalai Panbangred

Other Contributor(s)

Mahidol University
 Osaka University

Abstract

© 2015, Springer-Verlag Berlin Heidelberg. Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/43090

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Scopus 2016-2017