Publication: Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034
| dc.contributor.author | Bungonsiri Intra | en_US |
| dc.contributor.author | Jirayut Euanorasetr | en_US |
| dc.contributor.author | Takuya Nihira | en_US |
| dc.contributor.author | Watanalai Panbangred | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Osaka University | en_US |
| dc.date.accessioned | 2018-12-11T02:18:18Z | |
| dc.date.accessioned | 2019-03-14T08:04:09Z | |
| dc.date.available | 2018-12-11T02:18:18Z | |
| dc.date.available | 2019-03-14T08:04:09Z | |
| dc.date.issued | 2016-03-01 | en_US |
| dc.description.abstract | © 2015, Springer-Verlag Berlin Heidelberg. Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034. | en_US |
| dc.identifier.citation | Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-2760 | en_US |
| dc.identifier.doi | 10.1007/s00253-015-7142-8 | en_US |
| dc.identifier.issn | 14320614 | en_US |
| dc.identifier.issn | 01757598 | en_US |
| dc.identifier.other | 2-s2.0-84959162007 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/43090 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84959162007&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.subject | Immunology and Microbiology | en_US |
| dc.title | Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034 | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84959162007&origin=inward | en_US |