Publication: Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles
Issued Date
2010-08-01
Resource Type
ISSN
10985514
0022538X
0022538X
Other identifier(s)
2-s2.0-77954972691
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virology. Vol.84, No.16 (2010), 8253-8358
Suggested Citation
Jiraphan Junjhon, Thomas J. Edwards, Utaiwan Utaipat, Valorie D. Bowman, Heather A. Holdaway, Wei Zhang, Poonsook Keelapang, Chunya Puttikhunt, Rushika Perera, Paul R. Chipman, Watchara Kasinrerk, Prida Malasit, Richard J. Kuhn, Nopporn Sittisombut Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles. Journal of Virology. Vol.84, No.16 (2010), 8253-8358. doi:10.1128/JVI.00696-10 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29207
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Title
Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles
Abstract
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage. Copyright © 2010, American Society for Microbiology. All Rights Reserved.