Publication: Purification of an isoflavonoid 7-O-β-apiosyl-glucoside β-glycosidase and its substrates from Dalbergia nigrescens Kurz
Issued Date
2005-08-01
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ISSN
00319422
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2-s2.0-23944471715
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Mahidol University
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SCOPUS
Bibliographic Citation
Phytochemistry. Vol.66, No.16 (2005), 1880-1889
Suggested Citation
Phimonphan Chuankhayan, Yanling Hua, Jisnuson Svasti, Santi Sakdarat, Patrick A. Sullivan, James R. Ketudat Cairns Purification of an isoflavonoid 7-O-β-apiosyl-glucoside β-glycosidase and its substrates from Dalbergia nigrescens Kurz. Phytochemistry. Vol.66, No.16 (2005), 1880-1889. doi:10.1016/j.phytochem.2005.06.024 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16204
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Title
Purification of an isoflavonoid 7-O-β-apiosyl-glucoside β-glycosidase and its substrates from Dalbergia nigrescens Kurz
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Abstract
A β-glycosidase was purified from the seeds of Dalbergia nigescens Kurz based on its ability to hydrolyse p-nitrophenyl β-glucoside and β-fucoside. This enzyme did not hydrolyze various glycosidic substrates efficiently, so it was used to identify its own natural substrates. Two substrates were identified, isolated and their structures determined as: compound 1, dalpatein 7-O-β-d-apiofuranosyl-(1 → 6)-β-d- glucopyranoside and compound 2, 6,2′,4′,5′-tetramethoxy-7- hydroxy-7-O-β-d-apiofuranosyl-(1 → 6)-β-d-glucopyranoside (dalnigrein7-O-β-d-apiofuranosyl-(1 → 6)-β-d-glucopyranoside). The β-glycosidase removes the sugar from these glycosides as a disaccharide, despite its initial identification as a β-glucosidase and β-fucosidase. © 2005 Elsevier Ltd. All rights reserved.