Publication: The prediction of GAG and ENV gene on epidemiology of HIV-1 infection in Thailand
Issued Date
2014-01-01
Resource Type
ISSN
09757384
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2-s2.0-84907235537
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chemical and Pharmaceutical Research. Vol.6, No.7 (2014), 2327-2332
Suggested Citation
B. Sermsaksakul, S. Sonprasert, N. Horthongkham, P. Ammaranond The prediction of GAG and ENV gene on epidemiology of HIV-1 infection in Thailand. Journal of Chemical and Pharmaceutical Research. Vol.6, No.7 (2014), 2327-2332. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34910
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Title
The prediction of GAG and ENV gene on epidemiology of HIV-1 infection in Thailand
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Abstract
© 2014, Journal of Chemical and Pharmaceutical Research. All rights reserved. HIV was classified into 3 groups: group M, N and O. Group M is an important group for epidemiolody. In Thailand, CRF01_AE and B are the most common subtype. Because of heterogeneity HIV can recombine genes to another subtype and cause a new subtype. Forty-two plasma samples from HIV-infected patients were collected at Siriraj Hospital. Specific region of p17 (gag) and C2-V4 (env) genes of HIV were amplified by nested RT-PCR. PCR products were sequenced. Phylogenetic tree was constructed for epidemiological study. Results showed that phylogenetic analysis on env gene was found 100% of HIV subtype CRF01_AE (42/42). Whereas on gag (p17) gene, 69% and 31% were found in CRF01_AE and CRF15_01B subtype, respectively.