Publication: Complement-mediated muscle cell lysis: A possible mechanism of myonecrosis in anti-SRP associated necrotizing myopathy (ASANM)
Issued Date
2013-09-16
Resource Type
ISSN
18728421
01655728
01655728
Other identifier(s)
2-s2.0-84886773294
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Neuroimmunology. Vol.264, No.1-2 (2013), 65-70
Suggested Citation
Arada Rojana-udomsart, Chalermchai Mitrpant, Christine Bundell, Loren Price, Yue Bei Luo, Victoria Fabian, Steve D. Wilton, Peter Hollingsworth, Frank L. Mastaglia Complement-mediated muscle cell lysis: A possible mechanism of myonecrosis in anti-SRP associated necrotizing myopathy (ASANM). Journal of Neuroimmunology. Vol.264, No.1-2 (2013), 65-70. doi:10.1016/j.jneuroim.2013.08.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31869
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Title
Complement-mediated muscle cell lysis: A possible mechanism of myonecrosis in anti-SRP associated necrotizing myopathy (ASANM)
Abstract
The mechanism of necrotizing myopathy associated with antibodies to signal recognition particle (SRP) remains unclear. We investigated the effect of anti-SRP. +. serum and complement on cell viability in myoblast cultures. Cell viability was only slightly reduced by incubation with anti-SRP. +. serum compared with control serum. However, the addition of fresh complement resulted in a marked reduction in cell survival. Surface immunostaining for SRP, C3c and C5b-9 was demonstrated in cultures pre-incubated with anti-SRP. +. serum and complement, and in muscle biopsies from patients with myopathy. These findings provide further support for a complement-dependent antibody-mediated mechanism in anti-SRP associated myopathy. © 2013 Elsevier B.V.