Publication: Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis.
Issued Date
2005-06-01
Resource Type
ISSN
0894959X
Other identifier(s)
2-s2.0-24944457298
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Clinical laboratory science : journal of the American Society for Medical Technology. Vol.18, No.3 (2005), 139-144
Suggested Citation
Puntip Tantiwong, Gobchai Puavilai, Boonsong Ongphiphadhanakul, Pongamorn Bunnag, Chardpraorn Ngarmukos Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis.. Clinical laboratory science : journal of the American Society for Medical Technology. Vol.18, No.3 (2005), 139-144. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16335
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis.
Other Contributor(s)
Abstract
OBJECTIVE: To compare blood beta-hydroxybutyrate (beta-OHB) levels measured by reagent strip with serum ketone levels assessed by the nitroprusside reaction in diagnosing diabetic ketoacidosis (DKA). DESIGN: Prospective study of DKA in 19 Thai diabetic patients from September 2001 until August 2002. SETTING: A university hospital. PATIENTS: Nineteen patients with DKA and ten patients with metabolic acidosis from other causes. INTERVENTIONS: Capillary blood alpha-OHB was measured by a blood ketone meter (MediSense Optium). Concurrently, serum ketone was measured semiquantitatively by nitroprusside reaction (Ketostix, N-multistix, and Labstix). MAIN OUTCOME MEASURE: Sensitivity, specificity, and ROC curve of both methods in diagnosing DKA. RESULT: Mean age +/- SD of DKA patients was 45.6 +/- 16.95 years. Plasma glucose was 675.25 +/- 188.15 mg/dL, arterial blood pH 7.19 +/- 0.12, anion gap and serum bicarbonate 29.93 +/- 4.90 and 8 +/- 3.35 mmol/L. Serum ketone was moderately to markedly positive in most cases. Capillary beta-OHB ranged from 2.4 to >6 mmol/L. The sensitivity and specificity of serum ketone by nitroprusside reaction in diagnosing DKA were 95% and 100%. The sensitivity and specificity of capillary beta-OHB was 90% and 100% respectively. The areas under ROC curves of serum ketone and capillary beta-OHB were 0.975 and 0.950 (NS) respectively. CONCLUSION: Serum ketone and blood beta-OHB measurement are equally effective in diagnosing DKA among uncomplicated cases.