Publication: Bacterial Diversity and Phylogenetic Analysis of Type II Polyketide Synthase Gene from Manao-Pee Cave, Thailand
Issued Date
2018-07-03
Resource Type
ISSN
15210529
01490451
01490451
Other identifier(s)
2-s2.0-85042078419
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Mahidol University
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SCOPUS
Bibliographic Citation
Geomicrobiology Journal. Vol.35, No.6 (2018), 518-527
Suggested Citation
Apirak Wiseschart, Wuttichai Mhuanthong, Pattamaporn Thongkam, Sithichoke Tangphatsornruang, Duriya Chantasingh, Kusol Pootanakit Bacterial Diversity and Phylogenetic Analysis of Type II Polyketide Synthase Gene from Manao-Pee Cave, Thailand. Geomicrobiology Journal. Vol.35, No.6 (2018), 518-527. doi:10.1080/01490451.2017.1411993 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45737
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Title
Bacterial Diversity and Phylogenetic Analysis of Type II Polyketide Synthase Gene from Manao-Pee Cave, Thailand
Abstract
© 2018 Informa UK Limited, trading as Taylor & Francis Group. A subterranean limestone cave, Manao-Pee, was investigated for bacterial diversity and potential secondary metabolites production. Comparative 16 S rRNA analysis revealed that cave soil was highly dominated by Actinobacteria; whereas, Proteobacteria was highly abundant outside the cave. As Actinobacteria are biotechnologically valuable for their secondary metabolites, the diversity of the β-ketoacyl synthase (KSβ) was investigated. The results showed that the identified KSβhas 61–80% amino acid sequence identity to known sequences. Phylogenetic analysis placed some of the sequences in novel clades, suggesting the presence of novel KSβdomains. Thus, Manao-Pee cave is a promising habitat to discover potential novel bioactive compounds.