Publication:
Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate

2

Issued Date

1997-06-01

Resource Type

Article

ISSN

10399712

Other identifier(s)

2-s2.0-0031401760

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Biochemistry and Molecular Biology International. Vol.42, No.2 (1997), 399-408

Suggested Citation

Nuanchawee Wetprasit, Montri Chulavatnatol Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate. Biochemistry and Molecular Biology International. Vol.42, No.2 (1997), 399-408. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17893

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Title

Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate

Author(s)

Nuanchawee Wetprasit
 Montri Chulavatnatol

Other Contributor(s)

Mahidol University

Abstract

Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjunction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for α-anomer of galactoside with an aromatic residue.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

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https://repository.li.mahidol.ac.th/handle/123456789/17893

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Scopus 1991-2000