Publication: Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate
dc.contributor.author | Nuanchawee Wetprasit | en_US |
dc.contributor.author | Montri Chulavatnatol | en_US |
dc.contributor.other | Mahidol University | en_US |
dc.date.accessioned | 2018-07-04T07:40:22Z | |
dc.date.available | 2018-07-04T07:40:22Z | |
dc.date.issued | 1997-06-01 | en_US |
dc.description.abstract | Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjunction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for α-anomer of galactoside with an aromatic residue. | en_US |
dc.identifier.citation | Biochemistry and Molecular Biology International. Vol.42, No.2 (1997), 399-408 | en_US |
dc.identifier.issn | 10399712 | en_US |
dc.identifier.other | 2-s2.0-0031401760 | en_US |
dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/17893 | |
dc.rights | Mahidol University | en_US |
dc.rights.holder | SCOPUS | en_US |
dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031401760&origin=inward | en_US |
dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
dc.title | Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031401760&origin=inward | en_US |