Publication: Cross-species transmission of gibbon and orangutan hepatitis B virus to uPA/SCID mice with human hepatocytes
Issued Date
2011-06-01
Resource Type
ISSN
18727492
01681702
01681702
Other identifier(s)
2-s2.0-79956305144
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Mahidol University
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SCOPUS
Bibliographic Citation
Virus Research. Vol.158, No.1-2 (2011), 209-215
Suggested Citation
Pattaratida Sa-nguanmoo, Yasuhito Tanaka, Parntep Ratanakorn, Masaya Sugiyama, Shuko Murakami, Sunchai Payungporn, Angkana Sommanustweechai, Masashi Mizokami, Yong Poovorawan Cross-species transmission of gibbon and orangutan hepatitis B virus to uPA/SCID mice with human hepatocytes. Virus Research. Vol.158, No.1-2 (2011), 209-215. doi:10.1016/j.virusres.2011.04.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11547
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Title
Cross-species transmission of gibbon and orangutan hepatitis B virus to uPA/SCID mice with human hepatocytes
Abstract
To investigate the potential of cross-species transmission of non-human primate HBV to humans, severe combined immunodeficiency mice transgenic for urokinase-type plasminogen activator, in which the mouse liver has been engrafted with human hepatocytes, were inoculated with non-human primate HBV. HBV-DNA positive serum samples from a gibbon or orangutan were inoculated into 6 chimeric mice. HBV-DNA, hepatitis B surface antigen (HBsAg), and HB core-related antigen in sera and HBV cccDNA in liver were detectable in 2 of 3 mice each from the gibbon and orangutan. Likewise, applying immunofluorescence HBV core protein was only found in human hepatocytes expressing human albumin. The HBV sequences from mouse sera were identical to those from orangutan and gibbon sera determined prior to inoculation. In conclusion, human hepatocytes have been infected with gibbon/orangutan HBV. © 2011 Elsevier B.V.