Publication: Human sex determination by amelogenin padlock probes
Issued Date
2011-12-01
Resource Type
ISSN
1875175X
18751768
18751768
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2-s2.0-82455164317
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Mahidol University
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SCOPUS
Bibliographic Citation
Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011)
Suggested Citation
Piyarat Thaijaroen, Korapin Srisiri, Rapee Boonplueang, Nathinee Panvisavas Human sex determination by amelogenin padlock probes. Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011). doi:10.1016/j.fsigss.2011.09.110 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11411
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Title
Human sex determination by amelogenin padlock probes
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Abstract
In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future. © 2011 Elsevier Ireland Ltd.