Publication: Human sex determination by amelogenin padlock probes
| dc.contributor.author | Piyarat Thaijaroen | en_US |
| dc.contributor.author | Korapin Srisiri | en_US |
| dc.contributor.author | Rapee Boonplueang | en_US |
| dc.contributor.author | Nathinee Panvisavas | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-05-03T07:58:39Z | |
| dc.date.available | 2018-05-03T07:58:39Z | |
| dc.date.issued | 2011-12-01 | en_US |
| dc.description.abstract | In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future. © 2011 Elsevier Ireland Ltd. | en_US |
| dc.identifier.citation | Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011) | en_US |
| dc.identifier.doi | 10.1016/j.fsigss.2011.09.110 | en_US |
| dc.identifier.issn | 1875175X | en_US |
| dc.identifier.issn | 18751768 | en_US |
| dc.identifier.other | 2-s2.0-82455164317 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/11411 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=82455164317&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.subject | Medicine | en_US |
| dc.title | Human sex determination by amelogenin padlock probes | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=82455164317&origin=inward | en_US |