Publication:
Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method

Issued Date

2009-04-01

Resource Type

Article

ISSN

15324249
08905436

DOI

10.1080/08905430902880085

Other identifier(s)

2-s2.0-70449408460

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Food Biotechnology. Vol.23, No.2 (2009), 179-189

Suggested Citation

Duongdearn Suwanjinda, Kittipong Pala-or, Watanalai Panbangred Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method. Food Biotechnology. Vol.23, No.2 (2009), 179-189. doi:10.1080/08905430902880085 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27028

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Title

Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method

Author(s)

Duongdearn Suwanjinda
 Kittipong Pala-or
 Watanalai Panbangred

Other Contributor(s)

Mahidol University

Abstract

We developed a one-step method based on multiplex-overlapping polymerase chain reaction (mPCR) for species differentiation of Pediococcus acidilactici and P. pentosaceus as well as simultaneous detection of pediocin (ped) gene. The mPCR was performed in a single reaction tube with six primers consisting of four primers for identification of P. acidilactici and P. pentosaceus and two primers for pediocin gene amplification. We distinguished both species from nine other species of pediococci as well as several other lactic acid bacteria (LAB). The identification performed by this mPCR was confirmed by partial 16S rDNA sequencing of selected pediococcal and LAB isolates. This multiplex-overlapping PCR is a simple, rapid determination and reliable method for differentiation of both pediococcal species as well as determination of the ability to produce pediocin in a single PCR reaction. © Taylor & Francis Group, LLC.

Keyword(s)

Agricultural and Biological Sciences
 Biochemistry, Genetics and Molecular Biology
 Immunology and Microbiology

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URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/27028

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