Publication: Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method
2
Issued Date
2009-04-01
Resource Type
ISSN
15324249
08905436
08905436
Other identifier(s)
2-s2.0-70449408460
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Food Biotechnology. Vol.23, No.2 (2009), 179-189
Suggested Citation
Duongdearn Suwanjinda, Kittipong Pala-or, Watanalai Panbangred Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method. Food Biotechnology. Vol.23, No.2 (2009), 179-189. doi:10.1080/08905430902880085 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27028
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Title
Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method
Other Contributor(s)
Abstract
We developed a one-step method based on multiplex-overlapping polymerase chain reaction (mPCR) for species differentiation of Pediococcus acidilactici and P. pentosaceus as well as simultaneous detection of pediocin (ped) gene. The mPCR was performed in a single reaction tube with six primers consisting of four primers for identification of P. acidilactici and P. pentosaceus and two primers for pediocin gene amplification. We distinguished both species from nine other species of pediococci as well as several other lactic acid bacteria (LAB). The identification performed by this mPCR was confirmed by partial 16S rDNA sequencing of selected pediococcal and LAB isolates. This multiplex-overlapping PCR is a simple, rapid determination and reliable method for differentiation of both pediococcal species as well as determination of the ability to produce pediocin in a single PCR reaction. © Taylor & Francis Group, LLC.