Publication: Lighting E. coli cells as biological sensors for Cd2+
Issued Date
2001-09-06
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ISSN
01415492
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2-s2.0-0034871975
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Mahidol University
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SCOPUS
Bibliographic Citation
Biotechnology Letters. Vol.23, No.16 (2001), 1285-1291
Suggested Citation
Virapong Prachayasittikul, Chartchalerm Isarankura Na Ayudhya, Leif Bulow Lighting E. coli cells as biological sensors for Cd2+. Biotechnology Letters. Vol.23, No.16 (2001), 1285-1291. doi:10.1023/A:1010561120720 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26449
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Title
Lighting E. coli cells as biological sensors for Cd2+
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Abstract
Whole cells of E. coli expressing a chimeric cadmium-binding peptide fused to green fluorescent protein (CdBPGFP) were prepared and applied for the determination of cadmium. Construction of the structural gene was performed by inserting two synthetic oligonucleotides coding for four repeats of a Cd-binding peptide (His-Ser-Gln-Lys-Val-Phe) into the 5′-end of the GFPuv gene. Similarly, a hexahistidine-green fluorescent protein (his6GFPuv) was prepared and used as a reference in the determinations of heavy metals. The lowest concentrations of Cd, which activated the fluorescence, were 0.5 μM, 50 μM, and 0.5 mM for cells carrying CdBP4GFP, his6GFP and native GFP, respectively.