Publication:
Lighting E. coli cells as biological sensors for Cd2+

dc.contributor.authorVirapong Prachayasittikulen_US
dc.contributor.authorChartchalerm Isarankura Na Ayudhyaen_US
dc.contributor.authorLeif Bulowen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherLunds Universiteten_US
dc.date.accessioned2018-09-07T09:37:41Z
dc.date.available2018-09-07T09:37:41Z
dc.date.issued2001-09-06en_US
dc.description.abstractWhole cells of E. coli expressing a chimeric cadmium-binding peptide fused to green fluorescent protein (CdBPGFP) were prepared and applied for the determination of cadmium. Construction of the structural gene was performed by inserting two synthetic oligonucleotides coding for four repeats of a Cd-binding peptide (His-Ser-Gln-Lys-Val-Phe) into the 5′-end of the GFPuv gene. Similarly, a hexahistidine-green fluorescent protein (his6GFPuv) was prepared and used as a reference in the determinations of heavy metals. The lowest concentrations of Cd, which activated the fluorescence, were 0.5 μM, 50 μM, and 0.5 mM for cells carrying CdBP4GFP, his6GFP and native GFP, respectively.en_US
dc.identifier.citationBiotechnology Letters. Vol.23, No.16 (2001), 1285-1291en_US
dc.identifier.doi10.1023/A:1010561120720en_US
dc.identifier.issn01415492en_US
dc.identifier.other2-s2.0-0034871975en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/26449
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034871975&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemical Engineeringen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleLighting E. coli cells as biological sensors for Cd2+en_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034871975&origin=inwarden_US
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