Publication: Biomarker discovery in the biofilm-forming process of Burkholderia pseudomallei by mass-spectrometry
Issued Date
2019-04-01
Resource Type
ISSN
18728359
01677012
01677012
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2-s2.0-85061989437
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Microbiological Methods. Vol.159, (2019), 26-33
Suggested Citation
Piyapong Hadpanus, Permkun Permsirivisarn, Sitthiruk Roytrakul, Sumalee Tungpradabkul Biomarker discovery in the biofilm-forming process of Burkholderia pseudomallei by mass-spectrometry. Journal of Microbiological Methods. Vol.159, (2019), 26-33. doi:10.1016/j.mimet.2019.02.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50210
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Title
Biomarker discovery in the biofilm-forming process of Burkholderia pseudomallei by mass-spectrometry
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Abstract
© 2019 Elsevier B.V. A serious human infectious disease called Melioidosis is a result of Burkholderia pseudomallei infection. Treatment for infected individuals is difficult due to a wide range of ineffective antibiotics including a high level of antibiotic tolerance which has been known to be caused by biofilm production. However, biofilm forming processes of this bacterium are not well documented despite multiple-methodologies being applied. In this study, we utilized a proteomics strategy called whole cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (whole cell MALDI-TOF MS) to discover a potential biomarker relating biofilm forming in B. pseudomallei. The results presented a novel specific type of enzyme amylo-alpha-1, 6-glucosidase, which was demonstrated by a higher level of gene expression during the biofilm development. Our results also suggested a list of candidate markers that might be involved in this scenario. Eventually, this knowledge may expand valuable data to the biofilm study that may increase effective treatments for people infected with B. pseudomallei and possibly other antibiotic tolerant bacteria.