Publication:
Biomarker discovery in the biofilm-forming process of Burkholderia pseudomallei by mass-spectrometry

dc.contributor.authorPiyapong Hadpanusen_US
dc.contributor.authorPermkun Permsirivisarnen_US
dc.contributor.authorSitthiruk Roytrakulen_US
dc.contributor.authorSumalee Tungpradabkulen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand National Center for Genetic Engineering and Biotechnologyen_US
dc.date.accessioned2020-01-27T07:46:40Z
dc.date.available2020-01-27T07:46:40Z
dc.date.issued2019-04-01en_US
dc.description.abstract© 2019 Elsevier B.V. A serious human infectious disease called Melioidosis is a result of Burkholderia pseudomallei infection. Treatment for infected individuals is difficult due to a wide range of ineffective antibiotics including a high level of antibiotic tolerance which has been known to be caused by biofilm production. However, biofilm forming processes of this bacterium are not well documented despite multiple-methodologies being applied. In this study, we utilized a proteomics strategy called whole cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (whole cell MALDI-TOF MS) to discover a potential biomarker relating biofilm forming in B. pseudomallei. The results presented a novel specific type of enzyme amylo-alpha-1, 6-glucosidase, which was demonstrated by a higher level of gene expression during the biofilm development. Our results also suggested a list of candidate markers that might be involved in this scenario. Eventually, this knowledge may expand valuable data to the biofilm study that may increase effective treatments for people infected with B. pseudomallei and possibly other antibiotic tolerant bacteria.en_US
dc.identifier.citationJournal of Microbiological Methods. Vol.159, (2019), 26-33en_US
dc.identifier.doi10.1016/j.mimet.2019.02.011en_US
dc.identifier.issn18728359en_US
dc.identifier.issn01677012en_US
dc.identifier.other2-s2.0-85061989437en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/50210
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061989437&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleBiomarker discovery in the biofilm-forming process of Burkholderia pseudomallei by mass-spectrometryen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061989437&origin=inwarden_US

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