Publication: Sialic acid binding lectins from leaf of mulberry (Morus alba)
Issued Date
1998-12-31
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ISSN
01689452
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2-s2.0-0032585620
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Science. Vol.139, No.2 (1998), 141-148
Suggested Citation
Sunanta Ratanapo, Wayakorn Ngamjunyaporn, Montri Chulavatnatol Sialic acid binding lectins from leaf of mulberry (Morus alba). Plant Science. Vol.139, No.2 (1998), 141-148. doi:10.1016/S0168-9452(98)00185-X Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18231
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Title
Sialic acid binding lectins from leaf of mulberry (Morus alba)
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Abstract
Two new lectins, MLL 1 and MLL 2, were purified from the leaves of mulberry (Morus alba) by a procedure consisting of ammonium sulfate precipitation, affinity chromatography on a N-acetylgalactosamine-agarose column, gel filtration on a Sephacryl S-200 column, anion-exchange chromatography using a DEAE-Sephacel column and gel filtration on a Sephadex G-75 column. Both MLL 1 and MLL 2 were found to be glycoproteins with neutral sugar contents of 8.8 and 40%, respectively. They showed the same native molecular weight of 51 000. Each consisted of subunit with molecular weight of 16 500. They were highly specific for N-glycolylneuraminic acid.