Publication: A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis
Issued Date
2017-11-16
Resource Type
ISSN
14752697
0022149X
0022149X
Other identifier(s)
2-s2.0-85034600618
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Helminthology. (2017), 1-7
Suggested Citation
A. Ma, Y. Wang, X. L. Liu, H. M. Zhang, P. Eamsobhana, H. S. Yong, X. X. Gan A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis. Journal of Helminthology. (2017), 1-7. doi:10.1017/S0022149X17001080 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41385
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Title
A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis
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Abstract
Copyright © Cambridge University Press 2017 Human gnathostomiasis is an emerging food-borne parasitic disease caused by nematodes of the genus Gnathostoma. Currently, serological tests are commonly applied to support clinical diagnosis. In the present study, a simple and rapid filtration-based test, dot immune–gold filtration assay (DIGFA) was developed using a partially purified antigen of Gnathostoma third-stage larvae (L3). A total of 180 serum samples were tested to evaluate the diagnostic potential of DIGFA for gnathostomiasis. The diagnostic sensitivity and specificity were 96.7% (29/30) and 100% (25/25), respectively. The cross-reactivity with sera from other helminthiasis patients ranged from 0 to 4%, with an average of 1.6% (2/125). DIGFA using a partially purified L3 antigen was not only simple and rapid, but also more accurate than standard assays for the diagnosis of human gnathostomiasis. DIGFA may represent a promising tool for application in laboratories or in the field, without requiring any instrumentation.