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A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis

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dc.contributor.authorA. Maen_US
dc.contributor.authorY. Wangen_US
dc.contributor.authorX. L. Liuen_US
dc.contributor.authorH. M. Zhangen_US
dc.contributor.authorP. Eamsobhanaen_US
dc.contributor.authorH. S. Yongen_US
dc.contributor.authorX. X. Ganen_US
dc.contributor.otherZhejiang Academy of Medical Sciencesen_US
dc.contributor.otherChinese Center for Disease Control and Preventionen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of Malayaen_US
dc.date.accessioned2018-12-21T06:23:28Z
dc.date.accessioned2019-03-14T08:02:20Z
dc.date.available2018-12-21T06:23:28Z
dc.date.available2019-03-14T08:02:20Z
dc.date.issued2017-11-16en_US
dc.description.abstractCopyright © Cambridge University Press 2017 Human gnathostomiasis is an emerging food-borne parasitic disease caused by nematodes of the genus Gnathostoma. Currently, serological tests are commonly applied to support clinical diagnosis. In the present study, a simple and rapid filtration-based test, dot immune–gold filtration assay (DIGFA) was developed using a partially purified antigen of Gnathostoma third-stage larvae (L3). A total of 180 serum samples were tested to evaluate the diagnostic potential of DIGFA for gnathostomiasis. The diagnostic sensitivity and specificity were 96.7% (29/30) and 100% (25/25), respectively. The cross-reactivity with sera from other helminthiasis patients ranged from 0 to 4%, with an average of 1.6% (2/125). DIGFA using a partially purified L3 antigen was not only simple and rapid, but also more accurate than standard assays for the diagnosis of human gnathostomiasis. DIGFA may represent a promising tool for application in laboratories or in the field, without requiring any instrumentation.en_US
dc.identifier.citationJournal of Helminthology. (2017), 1-7en_US
dc.identifier.doi10.1017/S0022149X17001080en_US
dc.identifier.issn14752697en_US
dc.identifier.issn0022149Xen_US
dc.identifier.other2-s2.0-85034600618en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/41385
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85034600618&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleA filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasisen_US
dc.typeArticle in Pressen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85034600618&origin=inwarden_US

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