Expression of cell‐surface transferrin receptor following in vitro stimulation of peripheral blood lymphocytes in patients with β‐thalassaemia and iron‐deficiency anaemia

Abstract

Abstract: The ferritin concentration in peripheral blood lymphocyte extracts was measured in 10 normal subjects, 7 patients with homozygous β‐thalassaemia, and 5 patients with iron‐deficiency anaemia. The mean intracellular ferritin content was found increased in β‐thalassaemia and reduced in iron‐deficient patients. Incubation of mononuclear cells in phytohaemagglutinin medium led to an increase of DNA synthesis concomitant with an increased number of lymphocytes bearing transferrin receptor and interleukin‐2 receptor as measured by immunofluorescent technique. Although there was an immunological impairment of lymphocytes in patients with either iron depletion or iron loading compared to normal subjects, their ability to express transferrin receptor and interleukin‐2 receptor on their cell surface was normal. © Munksgaard 1990