Publication: Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument
Issued Date
2007-11-01
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ISSN
01252208
01252208
01252208
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2-s2.0-37148999653
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.90, No.11 (2007), 2429-2436
Suggested Citation
Sumonmal Uttayamakul, Sirirat Likanonsakul, Ruengpung Sutthent, Achara Chaovavanich Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument. Journal of the Medical Association of Thailand. Vol.90, No.11 (2007), 2429-2436. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24696
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Title
Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument
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Abstract
Objective: Commercial TaqMan real-time PCR reagent was modified and applied on Light Cylcer 1.2 for quantifying HIV-1 RNA in plasma and compared with the reference method; COBAS AmpliPrep/COBAS Amplicor HIV-1 monitor test version 1.5. Material and Method: Three hundred and eight frozen and fresh plasma samples were used for evaluation. Sequential specimens were also tested for follow-up cases. Results: The correlation between HIV-1 RNA values obtained by reference and modified method with automated and manual sample preparation were significant with r = 0.916 and 0.908 (p < 0.001, p < 0.001) respectively with similar agreement log of mean bias (0.5 versus 0.48). High degree of correlation and agreement were observed between the assays in blind fresh plasma, r = 0.953 (p < 0.001) with 0.15 log difference in HIV-1 RNA level. Among follow-up samples, both methods gave 100% concordant results. Conclusion: This modified protocol provided evidence for using modified commercial real-time PCR reagent for HIV-1 RNA quantitative detection as a monitoring tool for HIV/AIDS patients in Thailand.