Publication: Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D
Issued Date
2017-10-01
Resource Type
ISSN
18767753
18735061
18735061
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2-s2.0-85029441927
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.24, (2017), 21-24
Suggested Citation
Warunya Chakritbudsabong, Ladawan Sariya, Sirikron Pamonsupornvichit, Rassmeepen Pronarkngver, Somjit Chaiwattanarungruengpaisan, Joao N. Ferreira, Piyathip Setthawong, Praopilas Phakdeedindan, Mongkol Techakumphu, Theerawat Tharasanit, Sasitorn Rungarunlert Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D. Stem Cell Research. Vol.24, (2017), 21-24. doi:10.1016/j.scr.2017.08.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41752
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Title
Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D
Abstract
© 2017 The Authors Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.